Your browser doesn't support javascript.
loading
Repeatability of alkaline inorganic phosphate quantification in the skeletal muscle using 31P-magnetic resonance spectroscopy at 3 T.
Matias, Alexs A; Serviente, Corinna F; Decker, Stephen T; Erol, Muhammet Enes; Giuriato, Gaia; Le Fur, Yann; Nagarajan, Rajakumar; Bendahan, David; Layec, Gwenael.
Afiliación
  • Matias AA; Department of Kinesiology, University of Massachusetts at Amherst, Amherst, MA, USA.
  • Serviente CF; Department of Kinesiology, University of Massachusetts at Amherst, Amherst, MA, USA.
  • Decker ST; Diabetes & Metabolism Research Center, University of Utah, Salt Lake City, UT, USA.
  • Erol ME; Department of Kinesiology, University of Massachusetts at Amherst, Amherst, MA, USA.
  • Giuriato G; The Institute for Applied Life Sciences, University of Massachusetts at Amherst, Amherst, MA, USA.
  • Le Fur Y; Department of Kinesiology, University of Massachusetts at Amherst, Amherst, MA, USA.
  • Nagarajan R; School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE, USA.
  • Bendahan D; Department of Kinesiology, University of Massachusetts at Amherst, Amherst, MA, USA.
  • Layec G; School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE, USA.
NMR Biomed ; : e5255, 2024 Sep 03.
Article en En | MEDLINE | ID: mdl-39225116
ABSTRACT
The detection of a secondary inorganic phosphate (Pi) resonance, a possible marker of mitochondrial content in vivo, using phosphorus magnetic resonance spectroscopy (31P-MRS), poses technical challenges at 3 Tesla (T). Overcoming these challenges is imperative for the integration of this biomarker into clinical research. To evaluate the repeatability and reliability of measuring resting skeletal muscle alkaline Pi (Pialk) using with 31P-MRS at 3 T. After an initial set of experiments on five subjects to optimize the sequence, resting 31P-MRS of the quadriceps muscles were acquired on two visits (~4 days apart) using an intra-subjects design, from 13 sedentary to moderately active young male and female adults (22 ± 3 years old) within a whole-body 3 T MR system. Measurement variability attributed to changes in coil position, shimming procedure, and spectral analysis were quantified. 31P-MRS data were acquired with a 31P/-proton (1H) dual-tuned surface coil positioned on the quadriceps using a pulse-acquire sequence. Test-retest absolute and relative repeatability was analyzed using the coefficient of variation (CV) and intra-class correlation coefficients (ICC), respectively. After sequence parameter optimization, Pialk demonstrated high intra-subject repeatability (CV 10.6 ± 5.4%, ICC 0.80). Proximo-distal change in coil position along the length of the quadriceps introduced Pialk quantitation variability (CV 28 ± 5%), due to magnetic field inhomogeneity with more distal coil locations. In contrast, Pialk measurement variability due to repeated shims from the same muscle volume (0.40 ± 0.09mM; CV 6.6%), and automated spectral processing (0.37 ± 0.01mM; CV 2.3%), was minor. The quantification of Pialk in skeletal muscle via surface coil 31P-MRS at 3 T demonstrated excellent reproducibility. However, caution is advised against placing the coil at the distal part of the quadriceps to mitigate shimming inhomogeneity.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: NMR Biomed Asunto de la revista: DIAGNOSTICO POR IMAGEM / MEDICINA NUCLEAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: NMR Biomed Asunto de la revista: DIAGNOSTICO POR IMAGEM / MEDICINA NUCLEAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido