Absence of ATG9A and synaptophysin demixing on Rab5 mutation-induced giant endosomes.

Choi, Jiyoung; Wu, Yumei; Park, Daehun

Choi, Jiyoung; Wu, Yumei; Park, Daehun.

Afiliación

Choi J; Department of Medical and Biological Sciences, The Catholic University of Korea, Gyeonggi-Do, Bucheon, 14662, South Korea.
Wu Y; Department of Biotechnology, The Catholic University of Korea, Gyeonggi-Do, Bucheon, 14662, South Korea.
Park D; Departments of Neuroscience and of Cell Biology, HHMI, Program in Cellular Neuroscience, Neurodegeneration, and Repair, Yale School of Medicine, New Haven, CT, 06510, USA.

Mol Brain ; 17(1): 63, 2024 Sep 02.

Article en En | MEDLINE | ID: mdl-39223639

ABSTRACT

ABSTRACT

ATG9A is the only integral membrane protein among core autophagy-related (ATG) proteins. We previously found that ATG9A does not co-assemble into synaptophysin-positive vesicles, but rather, localizes to a distinct pool of vesicles within synapsin condensates in both fibroblasts and nerve terminals. The endocytic origin of these vesicles further suggests the existence of different intracellular sorting or segregation mechanisms for ATG9A and synaptophysin in cells. However, the precise underlying mechanism remains largely unknown. In this follow-up study, we investigated the endosomal localization of these two proteins by exploiting the advantages of a Rab5 mutant that induces the formation of enlarged endosomes. Notably, ATG9A and synaptophysin intermix perfectly and do not segregate on giant endosomes, indicating that the separation of these two proteins is not solely caused by the inherent properties of the proteins, but possibly by other unknown factors.

Asunto(s)

Proteínas Relacionadas con la Autofagia; Endosomas; Mutación; Sinaptofisina; Proteínas de Unión al GTP rab5; Endosomas/metabolismo; Mutación/genética; Sinaptofisina/metabolismo; Sinaptofisina/genética; Proteínas de Unión al GTP rab5/metabolismo; Proteínas de Unión al GTP rab5/genética; Animales; Proteínas Relacionadas con la Autofagia/metabolismo; Proteínas Relacionadas con la Autofagia/genética; Proteínas de la Membrana/metabolismo; Proteínas de la Membrana/genética; Humanos; Proteínas de Transporte Vesicular/metabolismo; Proteínas de Transporte Vesicular/genética; Ratones

Palabras clave

ATG9A; Endosomes; Liquidliquid phase separation; Synaptophysin; Vesicle clusters

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Endosomas / Sinaptofisina / Proteínas de Unión al GTP rab5 / Proteínas Relacionadas con la Autofagia / Mutación Límite: Animals / Humans Idioma: En Revista: Mol Brain Asunto de la revista: BIOLOGIA MOLECULAR / CEREBRO Año: 2024 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Reino Unido

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google