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Metabolic engineering of Escherichia coli for high-level production of benzyl acetate from glucose.
Ke, Qin; Liu, Chang; Zhuang, Yibin; Xue, Yaju; Cui, Zhanzhao; Zhang, Cuiying; Yin, Hua; Liu, Tao.
Afiliación
  • Ke Q; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China.
  • Liu C; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
  • Zhuang Y; National Center of Technology Innovation for Synthetic Biology, Tianjin, China.
  • Xue Y; Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin, China.
  • Cui Z; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
  • Zhang C; National Center of Technology Innovation for Synthetic Biology, Tianjin, China.
  • Yin H; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
  • Liu T; National Center of Technology Innovation for Synthetic Biology, Tianjin, China.
Microb Cell Fact ; 23(1): 238, 2024 Sep 03.
Article en En | MEDLINE | ID: mdl-39223542
ABSTRACT

BACKGROUND:

Benzyl acetate is an aromatic ester with a jasmine scent. It was discovered in plants and has broad applications in food, cosmetic, and pharmaceutical industries. Its current production predominantly relies on chemical synthesis. In this study, Escherichia coli was engineered to produce benzyl acetate.

RESULTS:

Two biosynthetic routes based on the CoA-dependent ß-oxidation pathway were constructed in E. coli for benzyl acetate production. In route I, benzoic acid pathway was extended to produce benzyl alcohol by combining carboxylic acid reductase and endogenous dehydrogenases and/or aldo-keto reductases in E. coli. Benzyl alcohol was then condensed with acetyl-CoA by the alcohol acetyltransferase ATF1 from yeast to form benzyl acetate. In route II, a plant CoA-dependent ß-oxidation pathway via benzoyl-CoA was assessed for benzyl alcohol and benzyl acetate production in E. coli. The overexpression of the phosphotransacetylase from Clostridium kluyveri (CkPta) further improved benzyl acetate production in E. coli. Two-phase extractive fermentation in situ was adopted and optimized for benzyl acetate production in a shake flask. The most optimal strain produced 3.0 ± 0.2 g/L benzyl acetate in 48 h by shake-flask fermentation.

CONCLUSIONS:

We were able to establish the whole pathway for benzyl acetate based on the CoA-dependent ß-oxidation in single strain for the first time. The highest titer for benzyl acetate produced from glucose by E. coli is reported. Moreover, cinnamyl acetate production as an unwanted by-product was very low. Results provided novel information regarding the engineering benzyl acetate production in microorganisms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escherichia coli / Ingeniería Metabólica / Glucosa Idioma: En Revista: Microb Cell Fact Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escherichia coli / Ingeniería Metabólica / Glucosa Idioma: En Revista: Microb Cell Fact Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido