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Drug resistance and genomic variations among Mycobacterium tuberculosis isolates from The Nile Delta, Egypt.
Soliman, May S; Hansen, Chungyi H; Hanafy, Mostafa; Shawky, Sherine; Rashed, Heba; Abdullah, Mohamed; Soliman, Noha Salah; Gad, Maha A; Khairat, Sahar; El-Kholy, Amani; Talaat, Adel M.
Afiliación
  • Soliman MS; Department of Clinical and Chemical Pathology, Kasr Al Aini, Faculty of Medicine, Cairo University, Giza, Egypt. may.mohmed@kasralainy.edu.eg.
  • Hansen CH; Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, USA.
  • Hanafy M; Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, USA.
  • Shawky S; Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
  • Rashed H; Microbiology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.
  • Abdullah M; Clinical Pathology Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
  • Soliman NS; Central Public Health Laboratories, Ministry of Health and Population, Cairo, Egypt.
  • Gad MA; Department of Clinical and Chemical Pathology, Kasr Al Aini, Faculty of Medicine, Cairo University, Giza, Egypt.
  • Khairat S; Department of Clinical and Chemical Pathology, Kasr Al Aini, Faculty of Medicine, Cairo University, Giza, Egypt.
  • El-Kholy A; Department of Clinical and Chemical Pathology, Kasr Al Aini, Faculty of Medicine, Cairo University, Giza, Egypt.
  • Talaat AM; Department of Clinical and Chemical Pathology, Kasr Al Aini, Faculty of Medicine, Cairo University, Giza, Egypt.
Sci Rep ; 14(1): 20401, 2024 09 02.
Article en En | MEDLINE | ID: mdl-39223176
ABSTRACT
Tuberculosis is a global public health concern. Earlier reports suggested the emergence of high rates of drug resistant tuberculosis in Egypt. This study included 102 isolates of Mycobacterium tuberculosis collected from two reference laboratories in Cairo and Alexandria. All clinical isolates were sub-cultured on Löwenstein-Jensen medium and analyzed using both BD BACTEC MGIT 960 SIRE Kit and standard diffusion disk assays to identify the antibiotic sensitivity profile. Extracted genomic DNA was subjected to whole genome sequencing (WGS) using Illumina platform. Isolates that belong to lineage 4 represented > 80%, while lineage 3 represented only 11% of the isolates. The percentage of drug resistance for the streptomycin, isoniazid, rifampicin and ethambutol were 31.0, 17.2, 19.5 and 20.7, respectively. Nearly 47.1% of the isolates were sensitive to the four anti-tuberculous drugs, while only one isolate was resistant to all four drugs. In addition, several new and known mutations were identified by WGS. High rates of drug resistance and new mutations were identified in our isolates. Tuberculosis control measures should focus on the spread of mono (S, I, R, E)- and double (S, E)-drug resistant strains present at higher rates throughout the whole Nile Delta, Egypt.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Tuberculosis Resistente a Múltiples Medicamentos / Secuenciación Completa del Genoma / Mycobacterium tuberculosis / Antituberculosos Límite: Adult / Female / Humans / Male / Middle aged País/Región como asunto: Africa Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Tuberculosis Resistente a Múltiples Medicamentos / Secuenciación Completa del Genoma / Mycobacterium tuberculosis / Antituberculosos Límite: Adult / Female / Humans / Male / Middle aged País/Región como asunto: Africa Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido