Your browser doesn't support javascript.
loading
Evaluating the accuracy of the MBT Lipid Xtract Kit for assessing colistin resistance in comparison to broth microdilution.
Lo, Calvin Ka-Fung; Ritchie, Gordon; Bilawka, Jennifer; Gowland, Leah; Chorlton, Samuel D; Jang, Willson; Matic, Nancy; Romney, Marc G; Stefanovic, Aleksandra; Lowe, Christopher F.
Afiliación
  • Lo CK; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Ritchie G; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Bilawka J; Division of Medical Microbiology and Virology, Providence Health Care, St. Paul's Hospital, Vancouver, British Columbia, Canada.
  • Gowland L; Division of Medical Microbiology and Virology, Providence Health Care, St. Paul's Hospital, Vancouver, British Columbia, Canada.
  • Chorlton SD; Division of Medical Microbiology and Virology, Providence Health Care, St. Paul's Hospital, Vancouver, British Columbia, Canada.
  • Jang W; BugSeq Bioinformatics, Vancouver, British Columbia, Canada.
  • Matic N; Division of Medical Microbiology and Virology, Providence Health Care, St. Paul's Hospital, Vancouver, British Columbia, Canada.
  • Romney MG; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Stefanovic A; Division of Medical Microbiology and Virology, Providence Health Care, St. Paul's Hospital, Vancouver, British Columbia, Canada.
  • Lowe CF; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
J Med Microbiol ; 73(9)2024 Sep.
Article en En | MEDLINE | ID: mdl-39222340
ABSTRACT
Colistin resistance testing methods such as broth microdilution (BMD) are time-consuming and labour intensive for clinical laboratories. MBT Lipid Xtract Kit on MALDI Biotyper Sirius System (Bruker, Billerica, MA, USA) utilizes lipidomic analysis to identify specific cell wall modifications associated with colistin resistance. We compared MBT to BMD (ComASP Colistin, Liofilchem) across 36 Gram-negative isolates (non-resistant MIC ≤2 µg ml-1, resistant MIC ≥4 µg ml-1). All samples were tested twice on MBT with discrepant results repeated before assessing categorical agreement between MBT and BMD. 44.4% (16/36) of isolates were colistin resistant via BMD. MBT Lipid Xtract had 80.6% agreement (29/36) with BMD, with 5/7 discrepancies corrected to match upon repeat testing. There was 100% agreement for Escherichia coli isolates (n=16). The whole-genome sequencing was completed on the two discrepant Klebsiella pneumoniae isolates, with variants within colistin resistance-associated loci identified (MIC 0.5 µg ml-1 arnC S30T, pmrB T246A, lapB N212T, lpxM S253G, crrB Q287K and MIC >16 µg ml-1 arnC S30T, pmrB R90insRN, pmrB T246A, pmrA E57G, lpxM S253G). Further evaluation, particularly for non-E. coli, of MBT is required prior to implementation in clinical laboratories.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Colistina / Farmacorresistencia Bacteriana / Antibacterianos Límite: Humans Idioma: En Revista: J Med Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Colistina / Farmacorresistencia Bacteriana / Antibacterianos Límite: Humans Idioma: En Revista: J Med Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido