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In Vivo Thoracic Dorsal Root Ganglia (DRG) Calcium Imaging and ECG Recording for Studying Peripheral Nerve Stimulation.
Li, Xia; Liu, Yun; Liu, Kun; Du, Longhua; Lv, Tao; Zhu, Bing; Gao, Xinyan.
Afiliación
  • Li X; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences.
  • Liu Y; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences.
  • Liu K; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences.
  • Du L; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences.
  • Lv T; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences.
  • Zhu B; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences.
  • Gao X; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences; gaoxy@mail.cintcm.ac.cn.
J Vis Exp ; (210)2024 Aug 16.
Article en En | MEDLINE | ID: mdl-39221953
ABSTRACT
The dorsal root ganglia (DRG), housing primary sensory neurons, transmit somatosensory and visceral afferent inputs to the dorsal horn of the spinal cord. They play a pivotal role in both physiological and pathological states, including neuropathic and visceral pain. In vivo calcium imaging of DRG enables real-time observation of calcium transients in single units or neuron ensembles. Accumulating evidence indicates that DRG neuronal activities induced by somatic stimulation significantly affect autonomic and visceral functions. While lumbar DRG calcium imaging has been extensively studied, thoracic segment DRG calcium imaging has been less explored due to surgical exposure and stereotaxic fixation challenges. Here, we utilized in vivo calcium imaging at the thoracic1 dorsal root ganglion (T1-DRG) to investigate changes in neuronal activity resulting from somatic stimulations of the forelimb. This approach is crucial for understanding the somato-cardiac reflex triggered by peripheral nerve stimulations (PENS), such as acupuncture. Notably, synchronization of cardiac function was observed and measured by electrocardiogram (ECG), with T-DRG neuronal activities, potentially establishing a novel paradigm for somato-visceral reflex in the thoracic segments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Electrocardiografía / Ganglios Espinales Límite: Animals Idioma: En Revista: J Vis Exp Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Electrocardiografía / Ganglios Espinales Límite: Animals Idioma: En Revista: J Vis Exp Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos