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Human Ovarian Surface Epithelium Organoids as a Platform to Study Tissue Regeneration.
Del Valle, Julieta S; Husetic, Azra; Diek, Dina; Rutgers, Laurens F; Asseler, Joyce D; Metzemaekers, Jeroen; van Mello, Norah M; Chuva de Sousa Lopes, Susana M.
Afiliación
  • Del Valle JS; Department of Anatomy and Embryology, Leiden University Medical Center; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center; j.s.del_valle@lumc.nl.
  • Husetic A; Department of Anatomy and Embryology, Leiden University Medical Center.
  • Diek D; Department of Anatomy and Embryology, Leiden University Medical Center.
  • Rutgers LF; Department of Anatomy and Embryology, Leiden University Medical Center.
  • Asseler JD; Department of Obstetrics and Gynaecology, Amsterdam University Medical Center; Centre of Expertise on Gender Dysphoria, Amsterdam UMC; Amsterdam Reproduction and Development Research Institute.
  • Metzemaekers J; Department of Gynaecology, Leiden University Medical Center.
  • van Mello NM; Department of Obstetrics and Gynaecology, Amsterdam University Medical Center; Centre of Expertise on Gender Dysphoria, Amsterdam UMC; Amsterdam Reproduction and Development Research Institute.
  • Chuva de Sousa Lopes SM; Department of Anatomy and Embryology, Leiden University Medical Center; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center; Ghent-Fertility and Stem Cell Team (G-FAST), Department of Reproductive Medicine, Ghent University Hospital; lopes@lumc.nl.
J Vis Exp ; (210)2024 Aug 16.
Article en En | MEDLINE | ID: mdl-39221930
ABSTRACT
The ovarian surface epithelium (OSE), the outermost layer of the ovary, undergoes rupture during each ovulation and plays a crucial role in ovarian wound healing while restoring ovarian integrity. Additionally, the OSE may serve as the source of epithelial ovarian cancers. Although the OSE regenerative properties have been well studied in mice, understanding the precise mechanism of tissue repair in the human ovary remains hampered by limited access to human ovaries and suitable in vitro culture protocols. Tissue-specific organoids, miniaturized in vitro models replicating both structural and functional aspects of the original organ, offer new opportunities for studying organ physiology, disease modeling, and drug testing. Here, we describe a method to isolate primary human OSE (hOSE) from whole ovaries and establish hOSE organoids. We include a morphological and cellular characterization showing heterogeneity between donors. Additionally, we demonstrate the capacity of this culture method to evaluate hormonal effects on OSE-organoid growth over a 2-week period. This method may enable the discovery of factors contributing to OSE regeneration and facilitate patient-specific drug screenings for malignant OSE.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Regeneración / Organoides Límite: Female / Humans Idioma: En Revista: J Vis Exp Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Regeneración / Organoides Límite: Female / Humans Idioma: En Revista: J Vis Exp Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos