Your browser doesn't support javascript.
loading
Spatial chemistry of citrus reveals molecules bactericidal to Candidatus Liberibacter asiaticus.
A Aksenov, Alexander; Blacutt, Alex; Ginnan, Nichole; Rolshausen, Philippe E; V Melnik, Alexey; Lotfi, Ali; C Gentry, Emily; Ramasamy, Manikandan; Zuniga, Cristal; Zengler, Karsten; Mandadi, Kranthi K; Dorrestein, Pieter C; Roper, M Caroline.
Afiliación
  • A Aksenov A; Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy & Pharmaceutical Sciences, University of California - San Diego, La Jolla, CA, USA. aaksenov@uconn.edu.
  • Blacutt A; Arome Science Inc., Farmington, CT, USA. aaksenov@uconn.edu.
  • Ginnan N; Department of Chemistry, University of Connecticut, Storrs, CT, USA. aaksenov@uconn.edu.
  • Rolshausen PE; Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.
  • V Melnik A; Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.
  • Lotfi A; One Health Microbiome Center, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA.
  • C Gentry E; Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
  • Ramasamy M; Department of Chemistry, University of Connecticut, Storrs, CT, USA.
  • Zuniga C; Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy & Pharmaceutical Sciences, University of California - San Diego, La Jolla, CA, USA.
  • Zengler K; Arome Science Inc., Farmington, CT, USA.
  • Mandadi KK; Department of Chemistry, University of Connecticut, Storrs, CT, USA.
  • Dorrestein PC; Department of Chemistry, University of Connecticut, Storrs, CT, USA.
  • Roper MC; Department of Chemistry, Virginia Tech, Blacksburg, VA, USA.
Sci Rep ; 14(1): 20306, 2024 09 02.
Article en En | MEDLINE | ID: mdl-39218988
ABSTRACT
Huanglongbing (HLB), associated with the psyllid-vectored phloem-limited bacterium, Candidatus Liberibacter asiaticus (CLas), is a disease threat to all citrus production worldwide. Currently, there are no sustainable curative or prophylactic treatments available. In this study, we utilized mass spectrometry (MS)-based metabolomics in combination with 3D molecular mapping to visualize complex chemistries within plant tissues to explore how these chemistries change in vivo in HLB-infected trees. We demonstrate how spatial information from molecular maps of branches and single leaves yields insight into the biology not accessible otherwise. In particular, we found evidence that flavonoid biosynthesis is disrupted in HLB-infected trees, and an increase in the polyamine, feruloylputrescine, is highly correlated with an increase in disease severity. Based on mechanistic details revealed by these molecular maps, followed by metabolic modeling, we formulated and tested the hypothesis that CLas infection either directly or indirectly converts the precursor compound, ferulic acid, to feruloylputrescine to suppress the antimicrobial effects of ferulic acid and biosynthetically downstream flavonoids. Using in vitro bioassays, we demonstrated that ferulic acid and bioflavonoids are indeed highly bactericidal to CLas, with the activity on par with a reference antibiotic, oxytetracycline, recently approved for HLB management. We propose these compounds should be evaluated as therapeutics alternatives to the antibiotics for HLB treatment. Overall, the utilized 3D metabolic mapping approach provides a promising methodological framework to identify pathogen-specific inhibitory compounds in planta for potential prophylactic or therapeutic applications.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Citrus / Antibacterianos Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Citrus / Antibacterianos Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido