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EtROP38 suppresses apoptosis of host cells infected with Eimeria tenella by inhibition of the p38MAPK pathway.
Duan, Bu-Ting; Zhang, Hao-Yu; Song, Zi-Hao; Han, Xiao-Yi; Cui, Kai-Ling; Xu, Tong; Zhang, Yu; Zhao, Yong-Juan; Lei, Xuan; Tan, Fan; Guo, Lu-Lu; Yang, Hui-Lin; Zhang, Li; Bai, Rui; Lv, Xiao-Ling; Zheng, Ming-Xue.
Afiliación
  • Duan BT; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Zhang HY; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Song ZH; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Han XY; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Cui KL; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Xu T; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Zhang Y; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Zhao YJ; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China; School of Food and Environment, Jinzhong College of Information, Taigu, Jinzhong, China.
  • Lei X; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Tan F; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Guo LL; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Yang HL; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Zhang L; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Bai R; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Lv XL; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China.
  • Zheng MX; College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Jinzhong, China. Electronic address: zhengmingxue288@163.com.
Vet Parasitol ; 331: 110296, 2024 Oct.
Article en En | MEDLINE | ID: mdl-39217762
ABSTRACT
Coccidiosis is an important parasitic disease that has serious adverse effects on the global poultry industry. The mechanism by which the pathogenic factors of Eimeria tenella damage host cells is unknown. Some kinases from the rhoptry compartment can regulate apoptosis of host cells. This study focused on revealing the role and critical nodes of E. tenella rhoptry protein (EtROP) 38 in controlling the apoptosis of host cells via the P38 mitogen-activated protein kinase (MAPK) signaling pathway. The cells were treated with EtROP38 protein, siRNA p38MAPK, or both. The rate of infection, apoptosis, and the dynamic changes in the expression and activation of key factor genes of the P38MAPK signaling pathway in host cells infected with E. tenella were measured. The results showed that the addition of EtROP38 and/or knockdown of the host cells p38 gene reduced the apoptosis rate of cecal epithelial cells (CECS), decreased the mRNA expressions of p38, p53, c-myc, c-fos, and c-jun and increased the expression of p65, decreased the protein expressions of c-myc, c-fos, and c-jun, decreased the p38 protein phosphorylation level, and increased the p65 protein phosphorylation level in CECS. When E. tenella was inoculated for 4-96 h, the addition of Et ROP38 and/or host cell p38 knockdown both increased the infection rate of host cells, and this effect was more pronounced with the addition of EtROP38 with the host cell p38 knockdown. These observations indicate that E. tenella can inhibits the activation of the p38MAPK signaling pathway in host cells via EtROP38, which suppresses apoptosis in host cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pollos / Eimeria tenella / Apoptosis / Proteínas Quinasas p38 Activadas por Mitógenos Límite: Animals Idioma: En Revista: Vet Parasitol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pollos / Eimeria tenella / Apoptosis / Proteínas Quinasas p38 Activadas por Mitógenos Límite: Animals Idioma: En Revista: Vet Parasitol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos