Paracrine signalling in breast cancer: Insights into the tumour endothelial phenotype.

Rass, Atarah; Eksteen, Carla; Engelbrecht, Anna-Mart

Rass, Atarah; Eksteen, Carla; Engelbrecht, Anna-Mart.

Afiliación

Rass A; Department of Physiological Sciences, Stellenbosch University, 2nd floor, Mike De Vries Building, Cnr. Merriman Ave & Bosman Street, Stellenbosch, South Africa. Electronic address: 19142609@sun.ac.za.
Eksteen C; Department of Physiological Sciences, Stellenbosch University, 2nd floor, Mike De Vries Building, Cnr. Merriman Ave & Bosman Street, Stellenbosch, South Africa.
Engelbrecht AM; Department of Physiological Sciences, Stellenbosch University, 2nd floor, Mike De Vries Building, Cnr. Merriman Ave & Bosman Street, Stellenbosch, South Africa; African Cancer Institute (ACI), Department of Global Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Stellenbosch, South Africa.

Acta Histochem ; 126(5-7): 152191, 2024 Oct.

Article en En | MEDLINE | ID: mdl-39216306

ABSTRACT

ABSTRACT

Tumour endothelial cells (TECs) are genetically and phenotypically distinct from their normal, healthy counterparts and provide various pro-tumourigenic effects. This study aimed to investigate the impact of conditioned media (CM) from non-tumourigenic MCF-12A breast epithelial cells as well as from MCF-7 and MDA-MB-231 breast cancer cells on human umbilical vein endothelial cells (HUVECs). Significant increases in cell viability were observed across all breast CM groups compared to controls, with notable differences between the MCF-12A, MCF-7, and MDA-MB-231 groups. Despite increased viability, no significant differences in MCM2 expression, a marker of cell proliferation, were detected. Morphological changes in HUVECs, including elongation, lumen formation, and branching, were more pronounced in breast cancer CM groups, especially in the MDA-MB-231 CM group. qPCR and Western blot analyses showed increased expression of TEC markers such as MDR1, LOX, and TEM8 in HUVECs treated with MCF-12A CM. The MCF-7 CM group significantly enhanced HUVEC migratory activity compared to MCF-12A CM, as evidenced by a scratch assay. These findings underscore distinct angiogenic responses elicited by non-tumourigenic and tumourigenic breast epithelial cells, with tumourigenic cells inducing a hyperactivated angiogenic response. The study highlights the differential effects of breast cancer cell paracrine signalling on endothelial cells and suggests the need for further investigation into TEC markers' role in both physiological and tumour angiogenesis.

Asunto(s)

Neoplasias de la Mama; Células Endoteliales de la Vena Umbilical Humana; Comunicación Paracrina; Humanos; Neoplasias de la Mama/metabolismo; Neoplasias de la Mama/patología; Femenino; Células Endoteliales de la Vena Umbilical Humana/metabolismo; Medios de Cultivo Condicionados/farmacología; Fenotipo; Células MCF-7; Proliferación Celular; Línea Celular Tumoral; Movimiento Celular; Neovascularización Patológica/metabolismo; Neovascularización Patológica/patología; Supervivencia Celular

Palabras clave

Angiogenesis; Breast cancer; Non-tumourigenic paracrine signalling; Tumour endothelial cells; Tumour microenvironment

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Comunicación Paracrina / Células Endoteliales de la Vena Umbilical Humana Límite: Female / Humans Idioma: En Revista: Acta Histochem Año: 2024 Tipo del documento: Article Pais de publicación: Alemania

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