Simple Analysis of Gel Images With IOCBIO Gel Software.
Bio Protoc
; 14(16): e5053, 2024 Aug 20.
Article
en En
| MEDLINE
| ID: mdl-39210956
ABSTRACT
Gel image analyses are often difficult to reproduce, as the most commonly used software, the ImageJ Gels plugin, does not automatically record any steps in the analysis process. This protocol provides detailed steps for image analysis using IOCBIO Gel software with western blot as an example; however, the protocol is applicable to all images obtained by electrophoresis, such as Southern blotting, northern blotting, and isoelectric focusing. IOCBIO Gel allows multiple sample analyses, linking the original image to all the operations performed on it, which can be stored in a central database or on a PC, ensuring ease of access and the possibility to perform corrections at each analysis stage. In addition, IOCBIO Gel is lightweight, with only minimal computer requirements. Key features ⢠Free and open-source software for analyzing gel images. ⢠Reproducibility. ⢠Can be used with images obtained by electrophoresis, such as western blotting, Southern blotting, isoelectric focusing, and more.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Bio Protoc
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estonia
Pais de publicación:
Estados Unidos