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Revealing the unseen: next generation sequencing for early detection of drug-resistant cytomegalovirus variants upon letermovir prophylaxis failure.
Nägele, Klaudia; Bättig, Veronika; Gosert, Rainer; Walti, Carla S; Prince, Spasenija Savic; Halter, Jörg; Mathews, Roby; Stühler, Claudia; Khanna, Nina; Leuzinger, Karoline.
Afiliación
  • Nägele K; Clinical Virology, University Hospital Basel, Basel, Switzerland.
  • Bättig V; Infectious Diseases & Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.
  • Gosert R; Clinical Virology, University Hospital Basel, Basel, Switzerland.
  • Walti CS; Infectious Diseases & Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.
  • Prince SS; Institute of Pathology, University Hospital Basel, Basel, Switzerland.
  • Halter J; Division of Hematology, University Hospital Basel, Basel, Switzerland.
  • Mathews R; Division of Hematology, University Hospital Basel, Basel, Switzerland.
  • Stühler C; Infection Biology Laboratory, Department of Biomedicine, University of Basel, Basel, Switzerland.
  • Khanna N; Infectious Diseases & Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.
  • Leuzinger K; Infection Biology Laboratory, Department of Biomedicine, University of Basel, Basel, Switzerland.
J Infect Dis ; 2024 Aug 30.
Article en En | MEDLINE | ID: mdl-39210611
ABSTRACT
In allogeneic hematopoietic cell transplant (HCT)-recipients, prophylactic management strategies are essential for preventing CMV-reactivation and associated disease. We report on a 63-year-old male patient with a D-/R+ CMV-serostatus, who showed ongoing low-level CMV-replication post-HCT despite receiving letermovir prophylaxis. Sanger-sequencing failed to detect drug resistance mutations (DRM) until CMV-pneumonitis developed, revealing a UL56-C325R-DRM linked to high-level letermovir resistance. Retrospective analysis with next-generation-sequencing (NGS) revealed the DRM at a low frequency of 6% two weeks prior to detection by Sanger-sequencing. This study highlights the importance of advanced NGS-methods for early detection of CMV-DRMs, allowing for faster adjustments in antiviral treatment strategies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Infect Dis Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Infect Dis Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos