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Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples.
Chen, Yannan; Chauhan, Shradha; Gong, Cheng; Dayton, Hannah; Xu, Cong; De La Cruz, Estanislao Daniel; Tsai, Yu-Young Wesley; Datta, Malika S; Rosoklija, Gorazd B; Dwork, Andrew J; Mann, J John; Boldrini, Maura; Leong, Kam W; Dietrich, Lars E P; Tomer, Raju.
Afiliación
  • Chen Y; Department of Biological Sciences, Columbia University, New York, NY, USA.
  • Chauhan S; Department of Biomedical Engineering, Columbia University, New York, NY, USA.
  • Gong C; Department of Biological Sciences, Columbia University, New York, NY, USA.
  • Dayton H; Department of Biological Sciences, Columbia University, New York, NY, USA.
  • Xu C; Department of Biomedical Engineering, Columbia University, New York, NY, USA.
  • De La Cruz ED; Department of Biological Sciences, Columbia University, New York, NY, USA.
  • Tsai YW; Department of Biomedical Engineering, Columbia University, New York, NY, USA.
  • Datta MS; Department of Biological Sciences, Columbia University, New York, NY, USA.
  • Rosoklija GB; Department of Biological Sciences, Columbia University, New York, NY, USA.
  • Dwork AJ; Mortimer B. Zuckerman Mind Brain and Behavior Institute, Columbia University, New York, NY, USA.
  • Mann JJ; Department of Biological Sciences, Columbia University, New York, NY, USA.
  • Boldrini M; Mortimer B. Zuckerman Mind Brain and Behavior Institute, Columbia University, New York, NY, USA.
  • Leong KW; Department of Psychiatry, Columbia University Irving Medical Center, New York State Psychiatric Institute, New York, NY, USA.
  • Dietrich LEP; Department of Psychiatry, Columbia University Irving Medical Center, New York State Psychiatric Institute, New York, NY, USA.
  • Tomer R; Department of Pathology and Cell Biology, Columbia University, New York, NY, USA.
Nat Biomed Eng ; 8(9): 1109-1123, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39209948
ABSTRACT
Light-sheet fluorescence microscopy (LSFM) is a widely used technique for imaging cleared tissue and living samples. However, high-performance LSFM systems are typically expensive and not easily scalable. Here we introduce a low-cost, scalable and versatile LSFM framework, which we named 'projected light-sheet microscopy' (pLSM), with high imaging performance and small device and computational footprints. We characterized the capabilities of pLSM, which repurposes readily available consumer-grade components, optimized optics, over-network control architecture and software-driven light-sheet modulation, by performing high-resolution mapping of cleared mouse brains and of post-mortem pathological human brain samples, and via the molecular phenotyping of brain and blood-vessel organoids derived from human induced pluripotent stem cells. We also report a method that leverages pLSM for the live imaging of the dynamics of sparsely labelled multi-layered bacterial pellicle biofilms at an air-liquid interface. pLSM can make high-resolution LSFM for biomedical applications more accessible, affordable and scalable.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Células Madre Pluripotentes Inducidas / Microscopía Fluorescente Límite: Animals / Humans Idioma: En Revista: Nat Biomed Eng Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Células Madre Pluripotentes Inducidas / Microscopía Fluorescente Límite: Animals / Humans Idioma: En Revista: Nat Biomed Eng Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido