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Comparison of four concentration methods of adenovirus, norovirus and rotavirus in tap water.
Elfellaki, Nouhaila; Berrouch, Salma; Biary, Abdelkader; Goïta, Simeon; Rafi, Houda; Lachkar, Hibatallah; Dehhani, Oussama; Rougemont, Alexis de; Bourlet, Thomas; Hafid, Jamal Eddine.
Afiliación
  • Elfellaki N; Laboratory of Bioresources and Food Safety, Faculty of Sciences and Technology, Cadi Ayyad University, Marrakech, Morocco.
  • Berrouch S; Laboratory of Bioresources and Food Safety, Faculty of Sciences and Technology, Cadi Ayyad University, Marrakech, Morocco; Higher School of Technology of El Kelâa des Sraghna, Cadi Ayyad University, El Kelâa des Sraghna, Morocco.
  • Biary A; Laboratory of Bioresources and Food Safety, Faculty of Sciences and Technology, Cadi Ayyad University, Marrakech, Morocco.
  • Goïta S; Laboratory of Bioresources and Food Safety, Faculty of Sciences and Technology, Cadi Ayyad University, Marrakech, Morocco.
  • Rafi H; Laboratory of Bioresources and Food Safety, Faculty of Sciences and Technology, Cadi Ayyad University, Marrakech, Morocco.
  • Lachkar H; Laboratory of Bioresources and Food Safety, Faculty of Sciences and Technology, Cadi Ayyad University, Marrakech, Morocco.
  • Dehhani O; Laboratory of Bioresources and Food Safety, Faculty of Sciences and Technology, Cadi Ayyad University, Marrakech, Morocco.
  • Rougemont A; National Reference Centre for Gastroenteritis Viruses, Laboratory of Virology, University Hospital of Dijon Bourgogne, France.
  • Bourlet T; Infectious Agents and Hygiene Department, University Hospital of Saint Etienne, Saint-Etienne, France.
  • Hafid JE; Laboratory of Bioresources and Food Safety, Faculty of Sciences and Technology, Cadi Ayyad University, Marrakech, Morocco. Electronic address: hfjamal@yahoo.fr.
J Virol Methods ; 330: 115013, 2024 Aug 28.
Article en En | MEDLINE | ID: mdl-39209160
ABSTRACT
Human enteric viruses, as adenovirus (HAdV), norovirus (HuNoV) and rotavirus (RVA) are significant causes of gastroenteritis associated with consumption of contaminated water worldwide. Various methods have been described for their detection and monitoring in water. The aim of this study was to compare the performance of four conditions for concentrating HAdV, HuNoV and RVA from water matrices, in order to develop a single protocol that could simultaneously concentrate all target viruses from tap water. The tested conditions were based on the adsorption-elution using electronegative filters, in which we evaluated cation-coated filtration by MgCl2 with or without acid rinse by H2SO4 and two elution buffers, namely NaOH and tris-glycine-beef extract. Genomic material was extracted and amplified by real-time PCR and real-time RT-PCR using commercial kits. Based on the statistical analysis of amplification results (cycles of quantification), the condition involving cation-coated filtration by MgCl2 using electronegative filters with acid rinse by H2SO4 combined with NaOH elution allowed efficient recovery of both HAdV, HuNoV and RVA from tap water compared to the other conditions. These findings confirm the effectiveness of the approach used to monitor three major enteric viruses in tap water.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Virol Methods Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Virol Methods Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos