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Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei.
Pang, Wei; Zhu, Jinkui; Yang, Kexin; Zhu, Xiaona; Zhou, Wei; Jiang, Linlin; Zhuang, Xuran; Liu, Yantong; Wei, Jianfeng; Lu, Xiaoxiang; Yin, Yao; Chen, Ziling; Xiang, Yangfei.
Afiliación
  • Pang W; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Zhu J; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Yang K; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Zhu X; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Zhou W; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Jiang L; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Zhuang X; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Liu Y; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Wei J; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Lu X; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Yin Y; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Chen Z; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Xiang Y; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; State Key Laboratory of Advanced Medical Materials and Devices, ShanghaiTech University, Shanghai 201210, China; Shanghai Clinical Research and Trial Center, Shanghai 201210, China. Electronic address: xiangyf@sh
Cell Stem Cell ; 2024 Aug 24.
Article en En | MEDLINE | ID: mdl-39208804
ABSTRACT
Brain organoids with nucleus-specific identities provide unique platforms for studying human brain development and diseases at a finer resolution. Despite its essential role in vital body functions, the medulla of the hindbrain has seen a lack of in vitro models, let alone models resembling specific medullary nuclei, including the crucial spinal trigeminal nucleus (SpV) that relays peripheral sensory signals to the thalamus. Here, we report a method to differentiate human pluripotent stem cells into region-specific brain organoids resembling the dorsal domain of the medullary hindbrain. Importantly, organoids specifically recapitulated the development of the SpV derived from the dorsal medulla. We also developed an organoid system to create the trigeminothalamic projections between the SpV and the thalamus by fusing these organoids, namely human medullary SpV-like organoids (hmSpVOs), with organoids representing the thalamus (hThOs). Our study provides a platform for understanding SpV development, nucleus-based circuit organization, and related disorders in the human brain.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cell Stem Cell Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cell Stem Cell Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos