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Phosphoglycerate Dehydrogenase Overexpression Inhibits Ferroptosis to Repress Calcification of Human Coronary Artery Vascular Smooth Muscle Cells via the P53/SLC7A11 Pathway.
Zou, Yuhai; Li, Dongdong; Guan, Ge; Liu, Wenting.
Afiliación
  • Zou Y; Department of Cardiology, General Hospital of Southern Theatre Command of PLA, Guangzhou, 510010, People's Republic of China.
  • Li D; Department of Cardiology, General Hospital of Southern Theatre Command of PLA, Guangzhou, 510010, People's Republic of China.
  • Guan G; Department of Cardiology, General Hospital of Southern Theatre Command of PLA, Guangzhou, 510010, People's Republic of China.
  • Liu W; Department of Otorhinolaryngology, Guangzhou First People's Hospital, Guangzhou, 510180, People's Republic of China.
Int J Gen Med ; 17: 3673-3687, 2024.
Article en En | MEDLINE | ID: mdl-39206267
ABSTRACT

Background:

Coronary artery calcification (CAC) is in almost all patients with coronary artery disease and requires more effective therapies. We aim to explore the effects of phosphoglycerate dehydrogenase (PHGDH) on CAC.

Methods:

We identified the differentially expressed genes through bioinformatic analysis and selected PHGDH for further verification. Human coronary artery smooth muscle cells (HCASMCs) cultured with calcifying medium were used as models of CAC in vitro. Erastin was administered to induce ferroptosis. We determined the cell viability by the cell count kit-8 assay. The alkaline phosphatase activity, calcium content, and the expression of glutathione were evaluated by the corresponding detection kits. The calcification level was detected by alizarin red staining. Then we performed Western blot to examine the expression of runt-related transcription factor 2, bone morphogenetic protein 2, cyclooxygenase 2, glutathione peroxidase 4, P53, and solute carrier family 7a member 11 (SLC7A11).

Results:

We acquired 201 differentially expressed genes and selected PHGDH to verify. In calcifying medium-induced HCASMCs, PHGDH overexpression increased the cell viability and decreased the alkaline phosphatase activity, calcium content, calcification level, and the expression of bone morphogenetic protein 2 and runt-related transcription factor 2. Additionally, we found higher levels of glutathione, glutathione peroxidase 4, and SLC7A11 and lower levels of cyclooxygenase 2 and P53 after up-regulating PHGDH. Erastin reversed the effects of PHGDH on calcification of HCASMCs.

Conclusion:

PHGDH overexpression suppresses the calcification level of HCASMCs by inhibiting ferroptosis through the P53/SLC7A11 signaling pathway, suggesting PHGDH as a promising therapeutic target of CAC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Gen Med Año: 2024 Tipo del documento: Article Pais de publicación: Nueva Zelanda
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Gen Med Año: 2024 Tipo del documento: Article Pais de publicación: Nueva Zelanda