Your browser doesn't support javascript.
loading
Pressure Cycling Technology Combined With MicroLC-SWATH Mass Spectrometry for the Analysis of Sex-Related Differences Between Male and Female Cerebella: A Promising Approach to Investigating Proteomics Differences in Psychiatric and Neurodegenerative Diseases.
Macur, Katarzyna; Roszkowska, Anna; Czaplewska, Paulina; Miekus-Purwin, Natalia; Klejbor, Ilona; Morys, Janusz; Baczek, Tomasz.
Afiliación
  • Macur K; Core Facility Laboratories, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
  • Roszkowska A; Department of Pharmaceutical Chemistry, Medical University of Gdansk, Gdansk, Poland.
  • Czaplewska P; Core Facility Laboratories, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
  • Miekus-Purwin N; Department of Pharmaceutical Chemistry, Medical University of Gdansk, Gdansk, Poland.
  • Klejbor I; Department of Anatomy, Institute of Medical Sciences, Jan Kochanowski University, Kielce, Poland.
  • Morys J; Department of Normal Anatomy, Pomeranian Medical University, Szczecin, Poland.
  • Baczek T; Department of Pharmaceutical Chemistry, Medical University of Gdansk, Gdansk, Poland.
Proteomics Clin Appl ; : e202400001, 2024 Aug 28.
Article en En | MEDLINE | ID: mdl-39205462
ABSTRACT

PURPOSE:

Pressure cycling technology (PCT) coupled with data-independent sequential window acquisition of all theoretical mass spectra (SWATH-MS) can be a powerful tool for identifying and quantifying biomarkers (e.g., proteins) in complex biological samples. Mouse models are frequently used in brain studies, including those focusing on different neurodevelopmental and psychiatric disorders. More and more pieces of evidence have suggested that sex-related differences in the brain impact the rates, clinical manifestations, and therapy outcomes of these disorders. However, sex-based differences in the proteomic profiles of mouse cerebella have not been widely investigated. EXPERIMENTAL

DESIGN:

In this pilot study, we evaluate the applicability of coupling PCT sample preparation with microLC-SWATH-MS analysis to map and identify differences in the proteomes of two female and two male mice cerebellum samples.

RESULTS:

We identified and quantified 174 proteins in mice cerebella. A comparison of the proteomic profiles revealed that the levels of 11 proteins in the female and male mice cerebella varied significantly. CONCLUSIONS AND CLINICAL RELEVANCE Although this study utilizes a small sample, our results indicate that the studied male and female mice cerebella possessed differing proteome compositions, mainly with respect to energy metabolism processes.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Proteomics Clin Appl Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Proteomics Clin Appl Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Alemania