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Aspergillus Fumigatus Spore Proteases Alter the Respiratory Mucosa Architecture and Facilitate Equine Herpesvirus 1 Infection.
Portaels, Joren; Van Crombrugge, Eline; Van Den Broeck, Wim; Lagrou, Katrien; Laval, Kathlyn; Nauwynck, Hans.
Afiliación
  • Portaels J; Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
  • Van Crombrugge E; Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
  • Van Den Broeck W; Department of Morphology, Medical Imaging, Orthopedics and Nutrition, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
  • Lagrou K; Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical Microbiology, 3000 Leuven, Belgium.
  • Laval K; Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
  • Nauwynck H; Department of Translational Physiology, Infectiology and Public Health, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
Viruses ; 16(8)2024 Jul 27.
Article en En | MEDLINE | ID: mdl-39205182
ABSTRACT
Numerous Aspergillus fumigatus (Af) airborne spores are inhaled daily by humans and animals due to their ubiquitous presence. The interaction between the spores and the respiratory epithelium, as well as its impact on the epithelial barrier function, remains largely unknown. The epithelial barrier protects the respiratory epithelium against viral infections. However, it can be compromised by environmental contaminants such as pollen, thereby increasing susceptibility to respiratory viral infections, including alphaherpesvirus equine herpesvirus type 1 (EHV-1). To determine whether Af spores disrupt the epithelial integrity and enhance susceptibility to viral infections, equine respiratory mucosal ex vivo explants were pretreated with Af spore diffusate, followed by EHV-1 inoculation. Spore proteases were characterized by zymography and identified using mass spectrometry-based proteomics. Proteases of the serine protease, metalloprotease, and aspartic protease groups were identified. Morphological analysis of hematoxylin-eosin (HE)-stained sections of the explants revealed that Af spores induced the desquamation of epithelial cells and a significant increase in intercellular space at high and low concentrations, respectively. The increase in intercellular space in the epithelium caused by Af spore proteases correlated with an increase in EHV-1 infection. Together, our findings demonstrate that Af spore proteases disrupt epithelial integrity, potentially leading to increased viral infection of the respiratory epithelium.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptido Hidrolasas / Aspergillus fumigatus / Esporas Fúngicas / Herpesvirus Équido 1 / Infecciones por Herpesviridae / Mucosa Respiratoria Límite: Animals Idioma: En Revista: Viruses Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptido Hidrolasas / Aspergillus fumigatus / Esporas Fúngicas / Herpesvirus Équido 1 / Infecciones por Herpesviridae / Mucosa Respiratoria Límite: Animals Idioma: En Revista: Viruses Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Suiza