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GhWRKY40 Interacts with an Asparaginase GhAPD6 Involved in Fiber Development in Upland Cotton (Gossypium hirsutum L.).
Zhang, Sujun; Cai, Xiao; Wei, Jingyan; Wang, Haitao; Liu, Cunjing; Li, Xinghe; Tang, Liyuan; Zhou, Xiaodong; Zhang, Jianhong.
Afiliación
  • Zhang S; Institute of Cotton, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, China.
  • Cai X; Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China.
  • Wei J; Institute of Cotton, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, China.
  • Wang H; Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China.
  • Liu C; National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
  • Li X; Institute of Cotton, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, China.
  • Tang L; Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China.
  • Zhou X; Institute of Cotton, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, China.
  • Zhang J; Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, China.
Genes (Basel) ; 15(8)2024 Jul 24.
Article en En | MEDLINE | ID: mdl-39202340
ABSTRACT
Fiber quality improvement is a primary goal in cotton breeding. Identification of fiber quality-related genes and understanding the underlying molecular mechanisms are essential prerequisites. Previously, studies determined that silencing the gene GhWRKY40 resulted in longer cotton fibers; however, both the underlying mechanisms and whether this transcription factor is additionally involved in the regulation of cotton fiber strength/fineness are unknown. In the current study, we verified that GhWRKY40 influences the fiber strength, fiber fineness, and fiber surface structure by using virus-induced gene silencing (VIGS). Potential proteins that may interact with the nucleus-localized GhWRKY40 were screened in a yeast two-hybrid (Y2H) nuclear-system cDNA library constructed from fibers at 0, 10, and 25 days post-anthesis (DPA) in two near-isogenic lines differing in fiber length and strength. An aspartyl protease/asparaginase-related protein, GhAPD6, was identified and confirmed by Y2H and split-luciferase complementation assays. The expression of GhAPD6 was approximately 30-fold higher in the GhWRKY40-VIGS lines at 10 DPA and aspartyl protease activity was significantly upregulated in the GhWRKY40-VIGS lines at 10-20 DPA. This study suggested that GhWRKY40 may interact with GhAPD6 to regulate fiber development in cotton. The results provide a theoretical reference for the selection and breeding of high-quality cotton fibers assisted by molecular technology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Regulación de la Expresión Génica de las Plantas / Gossypium / Fibra de Algodón Idioma: En Revista: Genes (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Regulación de la Expresión Génica de las Plantas / Gossypium / Fibra de Algodón Idioma: En Revista: Genes (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza