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Influence of Anticoagulants on the Dissociation of Cardiac Troponin Complex in Blood Samples.
Riabkova, Natalia S; Kogan, Alexander E; Katrukha, Ivan A; Vylegzhanina, Alexandra V; Bogomolova, Agnessa P; Alieva, Amina K; Pevzner, Dmitry V; Bereznikova, Anastasia V; Katrukha, Alexey G.
Afiliación
  • Riabkova NS; HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland.
  • Kogan AE; Department of Biochemistry, Faculty of Biology, Moscow State University, Leninskie Gory 1, str. 12, 119234 Moscow, Russia.
  • Katrukha IA; HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland.
  • Vylegzhanina AV; Department of Biochemistry, Faculty of Biology, Moscow State University, Leninskie Gory 1, str. 12, 119234 Moscow, Russia.
  • Bogomolova AP; HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland.
  • Alieva AK; Department of Biochemistry, Faculty of Biology, Moscow State University, Leninskie Gory 1, str. 12, 119234 Moscow, Russia.
  • Pevzner DV; HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland.
  • Bereznikova AV; HyTest Ltd., Intelligate 1, 6th Floor, Joukahaisenkatu 6, 20520 Turku, Finland.
  • Katrukha AG; Department of Biochemistry, Faculty of Biology, Moscow State University, Leninskie Gory 1, str. 12, 119234 Moscow, Russia.
Int J Mol Sci ; 25(16)2024 Aug 16.
Article en En | MEDLINE | ID: mdl-39201603
ABSTRACT
Immunodetection of cardiac isoforms of troponin I (cTnI) and troponin T (cTnT) in blood samples is widely used for the diagnosis of acute myocardial infarction. The cardiac troponin complex (ITC-complex), comprising cTnI, cTnT, and troponin C (TnC), makes up a large portion of troponins released into the bloodstream after the necrosis of cardiomyocytes. However, the stability of the ITC-complex has not been fully investigated. This study aimed to investigate the stability of the ITC-complex in blood samples. A native ITC-complex was incubated in buffer solutions, serum, and citrate, heparin, or EDTA plasma at various temperatures. Western blotting and gel filtration were performed, and troponins were detected using specific monoclonal antibodies. The ITC-complex dissociated at 37 °C in buffers with or without anticoagulants, in citrate, heparin, and EDTA plasmas, and in serum, into a binary cTnI-TnC complex (IC-complex) and free cTnT. In plasma containing heparin and EDTA, the IC-complex further dissociated into free TnC and cTnI. No dissociation was found at 4 °C or at room temperature (RT) in all matrices within 24 h except for EDTA plasma. After incubation at 37 °C in EDTA plasma and serum, dissociation was accompanied by proteolytic degradation of both cTnI and cTnT. The presence of anti-troponin autoantibodies in the sample impeded dissociation of the ITC-complex. The ITC-complex dissociates in vitro to form the IC-complex and free cTnT at 37 °C but is mostly stable at 4 °C or RT. Further dissociation of the IC-complex occurs at 37 °C in plasmas containing heparin and EDTA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Troponina I / Troponina T / Anticoagulantes Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Troponina I / Troponina T / Anticoagulantes Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Suiza