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Comparative Proteomic Identification of Ram Sperm before and after In Vitro Capacitation.
Chen, Ya-Le; Li, Chun-Yan; Wang, Peng-Hui; Wang, Ru; Zhuo, Xian; Zhang, Yan; Wang, Shi-Jia; Sun, Zhi-Peng; Chen, Jia-Hong; Cheng, Xiao; Zhang, Zi-Jun; Ren, Chun-Huan; Wang, Qiang-Jun.
Afiliación
  • Chen YL; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
  • Li CY; Yunnan Animal Science and Veterinary Institute, Kunming 650224, China.
  • Wang PH; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
  • Wang R; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
  • Zhuo X; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
  • Zhang Y; Yunnan Animal Science and Veterinary Institute, Kunming 650224, China.
  • Wang SJ; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
  • Sun ZP; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
  • Chen JH; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
  • Cheng X; Center of Agriculture Technology Cooperation and Promotion of Dingyuan County, Chuzhou 233200, China.
  • Zhang ZJ; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
  • Ren CH; College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
  • Wang QJ; Center of Agriculture Technology Cooperation and Promotion of Dingyuan County, Chuzhou 233200, China.
Animals (Basel) ; 14(16)2024 Aug 15.
Article en En | MEDLINE | ID: mdl-39199899
ABSTRACT
Ram sperm undergo a sequence of physiological and biochemical changes collectively termed as capacitation to perform oocyte fertilization. However, the protein changes induced by capacitation remain in need of further exploration. Thus, the present study investigated the comparative proteomic profiling in ram spermatozoa under non-capacitating (NC) and capacitating (CAP) conditions in vitro using a liquid chromatography-tandem mass spectrometry combined with tandem mass tag labeling strategy. As a results, 2050 proteins were identified and quantified; 348 of them were differentially abundant, with 280 of the proteins upregulated and 68 of the proteins downregulated between the CAP and NC spermatozoa, respectively. Functional enrichment analysis indicated that the differentially abundant proteins Prune Exopolyphosphatase 1, Galactose-1-Phosphate Uridylyltransferase, and ATP Citrate Lyase were strictly related to energy production and conversion, and Phosphoglycolate phosphatase, Glucosamine-6-Phosphate Deaminase 1 and 2 were related to metabolism, RNA processing, and vesicular transport pathways. Furthermore, the networks of protein-protein interaction indicated a strong interaction among these differential proteins in annotated pathways such as ubiquitin and transport metabolism. Our findings indicate that capacitation progress might be regulated through different pathways, providing insights into mechanisms involved in ram sperm capacitation and fertility.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Animals (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Animals (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza