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An Exosome-Laden Hydrogel Wound Dressing That Can Be Point-of-Need Manufactured in Austere and Operational Environments.
Wisdom, E Cate; Lamont, Andrew; Martinez, Hannah; Rockovich, Michael; Lee, Woojin; Gilchrist, Kristin H; Ho, Vincent B; Klarmann, George J.
Afiliación
  • Wisdom EC; USU Center for Biotechnology (4DBio3), Department of Radiology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
  • Lamont A; The Geneva Foundation, 917 Pacific Ave, Tacoma, WA 98402, USA.
  • Martinez H; USU Center for Biotechnology (4DBio3), Department of Radiology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
  • Rockovich M; The Geneva Foundation, 917 Pacific Ave, Tacoma, WA 98402, USA.
  • Lee W; The United States Air Force Academy, 2304 Cadet Drive, USAF Academy, CO 80840, USA.
  • Gilchrist KH; School of Medicine, Uniformed Service University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
  • Ho VB; The United States Naval Academy, 121 Blake Rd., Annapolis, MD 21402, USA.
  • Klarmann GJ; The United States Military Academy, 606 Thayer Rd., West Point, NY 10996, USA.
Bioengineering (Basel) ; 11(8)2024 Aug 08.
Article en En | MEDLINE | ID: mdl-39199762
ABSTRACT
Skin wounds often form scar tissue during healing. Early intervention with tissue-engineered materials and cell therapies may promote scar-free healing. Exosomes and extracellular vesicles (EV) secreted by mesenchymal stromal cells (MSC) are believed to have high regenerative capacity. EV bioactivity is preserved after lyophilization and storage to enable use in remote and typically resource-constrained environments. We developed a bioprinted bandage containing reconstituted EVs that can be fabricated at the point-of-need. An alginate/carboxymethyl cellulose (CMC) biomaterial ink was prepared, and printability and mechanical properties were assessed with rheology and compression testing. Three-dimensional printed constructs were evaluated for Young's modulus relative to infill density and crosslinking to yield material with stiffness suitable for use as a wound dressing. We purified EVs from human MSC-conditioned media and characterized them with nanoparticle tracking analysis and mass spectroscopy, which gave a peak size of 118 nm and identification of known EV proteins. Fluorescently labeled EVs were mixed to form bio-ink and bioprinted to characterize EV release. EV bandages were bioprinted on both a commercial laboratory bioprinter and a custom ruggedized 3D printer with bioprinting capabilities, and lyophilized EVs, biomaterial ink, and thermoplastic filament were deployed to an austere Arctic environment and bioprinted. This work demonstrates that EVs can be bioprinted with an alginate/CMC hydrogel and released over time when in contact with a skin-like substitute. The technology is suitable for operational medical applications, notably in resource-limited locations, including large-scale natural disasters, humanitarian crises, and combat zones.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioengineering (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioengineering (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza