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In Vitro Modulation of Autophagy by New Antioxidant Nitrones as a Potential Therapeutic Approach for the Treatment of Ischemic Stroke.
Izquierdo-Bermejo, Sara; Chamorro, Beatriz; Martín-de-Saavedra, María Dolores; Lobete, Miguel; López-Muñoz, Francisco; Marco-Contelles, José; Oset-Gasque, María Jesús.
Afiliación
  • Izquierdo-Bermejo S; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Complutense University of Madrid, Plaza Ramón y Cajal s/n, Ciudad Universitaria, 28040 Madrid, Spain.
  • Chamorro B; Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, 28040 Madrid, Spain.
  • Martín-de-Saavedra MD; Faculty of Health Sciences-HM Hospitals, Camilo José Cela University, Villafranca del Castillo, 28692 Madrid, Spain.
  • Lobete M; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Complutense University of Madrid, Plaza Ramón y Cajal s/n, Ciudad Universitaria, 28040 Madrid, Spain.
  • López-Muñoz F; Faculty of Health Sciences-HM Hospitals, Camilo José Cela University, Villafranca del Castillo, 28692 Madrid, Spain.
  • Marco-Contelles J; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Complutense University of Madrid, Plaza Ramón y Cajal s/n, Ciudad Universitaria, 28040 Madrid, Spain.
  • Oset-Gasque MJ; Instituto de Investigación Sanitaria del Hospital Clínico San Carlos, 28040 Madrid, Spain.
Antioxidants (Basel) ; 13(8)2024 Aug 03.
Article en En | MEDLINE | ID: mdl-39199193
ABSTRACT
Stroke is a leading cause of death worldwide, yet current therapeutic strategies remain limited. Among the neuropathological events underlying this disease are multiple cell death signaling cascades, including autophagy. Recent interest has focused on developing agents that target molecules involved in autophagy to modulate this process under pathological conditions. This study aimed to analyze the role of autophagy in cell death induced by an in vitro ischemia-reperfusion (IR) model and to determine whether nitrones, known for their neuroprotective and antioxidant effects, could modulate this process. We focused on key proteins involved in different phases of autophagy HIF-1α, BNIP3, and BECN1 for induction and nucleation, LC3 for elongation, and p62 for degradation. Our findings confirmed that the IR model promotes autophagy, initially via HIF-1α activation. Additionally, the neuroprotective effect of three of the selected synthetic nitrones (quinolylnitrones QN6 and QN23, and homo-bis-nitrone HBN6) partially derives from their antiautophagic properties, demonstrated by a downregulation of the expression of molecular markers involved in various phases of autophagy. In contrast, the neuroprotective power of cholesteronitrone ChN2 seems to derive from its promoting effects on the initial phases of autophagy, which could potentially help inhibit other forms of cell death. These results underscore the importance of autophagy modulation in neuroprotection, highlighting the potential of inhibiting prodeath autophagy and promoting prosurvival autophagy as promising therapeutic approaches in treating ischemic stroke clinically.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antioxidants (Basel) Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antioxidants (Basel) Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza