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An integrated single-cell reference atlas of the human endometrium.
Marecková, Magda; Garcia-Alonso, Luz; Moullet, Marie; Lorenzi, Valentina; Petryszak, Robert; Sancho-Serra, Carmen; Oszlanczi, Agnes; Icoresi Mazzeo, Cecilia; Wong, Frederick C K; Kelava, Iva; Hoffman, Sophie; Krassowski, Michal; Garbutt, Kurtis; Gaitskell, Kezia; Yancheva, Slaveya; Woon, Ee Von; Male, Victoria; Granne, Ingrid; Hellner, Karin; Mahbubani, Krishnaa T; Saeb-Parsy, Kourosh; Lotfollahi, Mohammad; Prigmore, Elena; Southcombe, Jennifer; Dragovic, Rebecca A; Becker, Christian M; Zondervan, Krina T; Vento-Tormo, Roser.
Afiliación
  • Marecková M; Wellcome Sanger Institute, Cambridge, UK.
  • Garcia-Alonso L; Oxford Endometriosis Care Centre, Nuffield Department of Women's & Reproductive Health, University of Oxford, Oxford, UK.
  • Moullet M; Wellcome Sanger Institute, Cambridge, UK.
  • Lorenzi V; Wellcome Sanger Institute, Cambridge, UK.
  • Petryszak R; Wellcome Sanger Institute, Cambridge, UK.
  • Sancho-Serra C; European Bioinformatics Institute-European Molecular Biology Laboratory, Cambridge, UK.
  • Oszlanczi A; Wellcome Sanger Institute, Cambridge, UK.
  • Icoresi Mazzeo C; Wellcome Sanger Institute, Cambridge, UK.
  • Wong FCK; Wellcome Sanger Institute, Cambridge, UK.
  • Kelava I; Wellcome Sanger Institute, Cambridge, UK.
  • Hoffman S; Wellcome Sanger Institute, Cambridge, UK.
  • Krassowski M; Wellcome Sanger Institute, Cambridge, UK.
  • Garbutt K; Wellcome Sanger Institute, Cambridge, UK.
  • Gaitskell K; Oxford Endometriosis Care Centre, Nuffield Department of Women's & Reproductive Health, University of Oxford, Oxford, UK.
  • Yancheva S; Centre for Human Genetics, University of Oxford, Oxford, UK.
  • Woon EV; Oxford Endometriosis Care Centre, Nuffield Department of Women's & Reproductive Health, University of Oxford, Oxford, UK.
  • Male V; Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
  • Granne I; Department of Cellular Pathology, John Radcliffe Hospital, Oxford, UK.
  • Hellner K; Department of Cellular Pathology, John Radcliffe Hospital, Oxford, UK.
  • Mahbubani KT; Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Imperial College London, London, UK.
  • Saeb-Parsy K; The Fertility Centre, Chelsea and Westminster Hospital, London, UK.
  • Lotfollahi M; Department of Metabolism, Digestion and Reproduction, Institute of Reproductive and Developmental Biology, Imperial College London, London, UK.
  • Prigmore E; Oxford Endometriosis Care Centre, Nuffield Department of Women's & Reproductive Health, University of Oxford, Oxford, UK.
  • Southcombe J; Oxford Endometriosis Care Centre, Nuffield Department of Women's & Reproductive Health, University of Oxford, Oxford, UK.
  • Dragovic RA; Department of Haematology, University of Cambridge, Cambridge, UK.
  • Becker CM; Cambridge Biorepository for Translational Medicine (CBTM), NIHR Cambridge Biomedical Research Centre, Cambridge, UK.
  • Zondervan KT; Cambridge Biorepository for Translational Medicine (CBTM), NIHR Cambridge Biomedical Research Centre, Cambridge, UK.
  • Vento-Tormo R; Department of Surgery, University of Cambridge, Cambridge, UK.
Nat Genet ; 56(9): 1925-1937, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39198675
ABSTRACT
The complex and dynamic cellular composition of the human endometrium remains poorly understood. Previous endometrial single-cell atlases profiled few donors and lacked consensus in defining cell types. We introduce the Human Endometrial Cell Atlas (HECA), a high-resolution single-cell reference atlas (313,527 cells) combining published and new endometrial single-cell transcriptomics datasets of 63 women with and without endometriosis. HECA assigns consensus and identifies previously unreported cell types, mapped in situ using spatial transcriptomics and validated using a new independent single-nuclei dataset (312,246 nuclei, 63 donors). In the functionalis, we identify intricate stromal-epithelial cell coordination via transforming growth factor beta (TGFß) signaling. In the basalis, we define signaling between fibroblasts and an epithelial population expressing progenitor markers. Integration of HECA with large-scale endometriosis genome-wide association study data pinpoints decidualized stromal cells and macrophages as most likely dysregulated in endometriosis. The HECA is a valuable resource for studying endometrial physiology and disorders, and for guiding microphysiological in vitro systems development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Endometriosis / Endometrio / Análisis de la Célula Individual Límite: Female / Humans Idioma: En Revista: Nat Genet Asunto de la revista: GENETICA MEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Endometriosis / Endometrio / Análisis de la Célula Individual Límite: Female / Humans Idioma: En Revista: Nat Genet Asunto de la revista: GENETICA MEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos