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An unexpected IgE anti-receptor binding domain response following natural infection and different types of SARS-CoV-2 vaccines.
Portilho, Amanda Izeli; Silva, Valéria Oliveira; Da Costa, Hernan Hermes Monteiro; Yamashiro, Rosemeire; de Oliveira, Isabela Penteriche; de Campos, Ivana Barros; Prudencio, Carlos Roberto; Matsuda, Elaine Monteiro; de Macedo Brígido, Luís Fernando; De Gaspari, Elizabeth.
Afiliación
  • Portilho AI; Immunology Center, Adolfo Lutz Institute, Av Dr Arnaldo, 355, 11th floor, room 1116, Pacaembu, São Paulo, SP, 01246-902, Brazil.
  • Silva VO; Post Graduate Program Interunits in Biotechnology, University of São Paulo, São Paulo, SP, Brazil.
  • Da Costa HHM; Virology Center, Adolfo Lutz Institute, São Paulo, SP, Brazil.
  • Yamashiro R; Post Graduate Program in Public Health Surveillance, Disease Control Coordination, São Paulo, SP, Brazil.
  • de Oliveira IP; Immunology Center, Adolfo Lutz Institute, Av Dr Arnaldo, 355, 11th floor, room 1116, Pacaembu, São Paulo, SP, 01246-902, Brazil.
  • de Campos IB; Post Graduate Program Interunits in Biotechnology, University of São Paulo, São Paulo, SP, Brazil.
  • Prudencio CR; Immunology Center, Adolfo Lutz Institute, Av Dr Arnaldo, 355, 11th floor, room 1116, Pacaembu, São Paulo, SP, 01246-902, Brazil.
  • Matsuda EM; Santo André Healthy Secretary, Santo André, SP, Brazil.
  • de Macedo Brígido LF; Santo André Regional Center, Adolfo Lutz Institute, Santo André, SP, Brazil.
  • De Gaspari E; Immunology Center, Adolfo Lutz Institute, Av Dr Arnaldo, 355, 11th floor, room 1116, Pacaembu, São Paulo, SP, 01246-902, Brazil.
Sci Rep ; 14(1): 20003, 2024 08 28.
Article en En | MEDLINE | ID: mdl-39198569
ABSTRACT
Humoral response to SARS-CoV-2 has been studied, predominantly the classical IgG and its subclasses. Although IgE antibodies are typically specific to allergens or parasites, a few reports describe their production in response to SARS-CoV-2 and other viruses. Here, we investigated IgE specific to receptor binding domain (RBD) of SARS-CoV-2 in a Brazilian cohort following natural infection and vaccination. Samples from 59 volunteers were assessed after infection (COVID-19), primary immunization with vectored (ChAdOx1) or inactivated (CoronaVac) vaccines, and booster immunization with mRNA (BNT162b2) vaccine. Natural COVID-19 induced IgE, but vaccination increased its levels. Subjects vaccinated with two doses of ChAdOx1 exhibited a more robust response than those immunized with two doses of CoronaVac; however, after boosting with BNT162b2, all groups presented similar IgE levels. IgE showed intermediate-to-high avidity, especially after the booster vaccine. We also found IgG4 antibodies, mainly after the booster, and they moderately correlated with IgE. ELISA results were confirmed by control assays, using IgG depletion by protein G and lack of reactivity with heterologous antigen. In our cohort, no clinical data could be associated with the IgE response. We advocate for further research on IgE and its role in viral immunity, extending beyond allergies and parasitic infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina E / Inmunoglobulina G / Glicoproteína de la Espiga del Coronavirus / Vacunas contra la COVID-19 / SARS-CoV-2 / COVID-19 / Anticuerpos Antivirales Límite: Adult / Female / Humans / Male / Middle aged País/Región como asunto: America do sul / Brasil Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoglobulina E / Inmunoglobulina G / Glicoproteína de la Espiga del Coronavirus / Vacunas contra la COVID-19 / SARS-CoV-2 / COVID-19 / Anticuerpos Antivirales Límite: Adult / Female / Humans / Male / Middle aged País/Región como asunto: America do sul / Brasil Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido