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Astragalus Polysaccharide improves immunogenicity of influenza vaccine as well as modulate gut microbiota in BALB/c mice.
Wan, Chuanqi; Yan, Sijing; Lu, Rufeng; Zhu, Chen; Yang, Yang; Wu, Xiaowei; Yu, Zhihong; Jiang, Mei; Peng, Wei; Song, Wenwen; Wu, Haibo; Fang, Bangjiang; He, Yuzhou.
Afiliación
  • Wan C; Department of Emergency, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, Xuhui, China.
  • Yan S; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zh
  • Lu R; Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
  • Zhu C; Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
  • Yang Y; Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
  • Wu X; Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
  • Yu Z; Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
  • Jiang M; Tianjin University of Traditional Chinese Medicine, Tianjin, China.
  • Peng W; Department of Emergency, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, Xuhui, China.
  • Song W; Shenzhen Kang Jian Mai de Technology Co., Ltd., Shenzhen, China.
  • Wu H; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zh
  • Fang B; Department of Emergency, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, Xuhui, China. Electronic address: fangbji@163.com.
  • He Y; The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. Electronic address: hyzxy1995@126.com.
Microb Pathog ; 195: 106893, 2024 Oct.
Article en En | MEDLINE | ID: mdl-39197333
ABSTRACT

BACKGROUND:

Vaccination is the best way to prevent influenza virus infection, and insufficient antibodies make it difficult to resist influenza virus invasion. Astragalus Polysaccharide (APS) has a boosting effect on immunity, so we evaluate the effect of APS as an immune adjuvant for H1N1 influenza vaccines in this study.

METHODS:

The mice were immunized twice with influenza A (H1N1) vaccine and APS. Subsequently, the serum antibody levels were assessed using enzyme-linked immunosorbent assay (ELISA). The frequency of peripheral immune T cells was determined by flow cytometry. Following this, the immunized mice were exposed to a lethal dose of the virus, and changes in body weight and survival rates were recorded. Hematoxylin-eosin staining was employed to observe pathological alterations in lung and intestinal tissues. Western blot analysis was conducted to detect the expression of intestinal barrier function proteins (Occludin and Claudin-1). ELISA was utilized to measure the expression level of serum inflammatory cytokine TNF-α. Fresh mouse feces were collected after the initial immunization as well as after viral infection for 16S rRNA analysis aimed at detecting alterations in gut microbiota.

RESULTS:

Compared to the Hemagglutinin (HA) group, the APS group demonstrated higher levels of immunoglobulin G (IgG), IgG1, and IgG3, as well as neutralizing antibody levels. Additionally, it increased the frequency of CD8+ cells to enhance resistance against lethal infection. On day 14 post-infection, the high-dose APS group exhibited a higher survival rate (71.40 %) compared to the HA group (14.28 %), along with faster weight recovery. Furthermore, APS was found to ameliorate alveolar damage in lung tissue and rectify intestinal structural disorder. It also upregulated the expression levels of tight junction proteins Occludin and Claudin-1 in intestinal tissue while reducing serum TNF-α expression levels. In addition, populations of Colidextribacter, Peptococcaceae, and Ruminococcaceae were the dominant gut microbiota in the APS group after viral infection.

CONCLUSION:

APS has an immune-enhancing effect and is expected to be a novel adjuvant in the H1N1 influenza vaccine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Vacunas contra la Influenza / Adyuvantes Inmunológicos / Infecciones por Orthomyxoviridae / Planta del Astrágalo / Subtipo H1N1 del Virus de la Influenza A / Microbioma Gastrointestinal / Ratones Endogámicos BALB C / Anticuerpos Antivirales Límite: Animals Idioma: En Revista: Microb Pathog Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Vacunas contra la Influenza / Adyuvantes Inmunológicos / Infecciones por Orthomyxoviridae / Planta del Astrágalo / Subtipo H1N1 del Virus de la Influenza A / Microbioma Gastrointestinal / Ratones Endogámicos BALB C / Anticuerpos Antivirales Límite: Animals Idioma: En Revista: Microb Pathog Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido