Your browser doesn't support javascript.
loading
Repeat Next-Generation Sequencing (15-Gene Panel) in Unifocal, Synchronous, and Metachronous Non-Small-Cell Lung Cancer-A Single-Center Experience.
Kuang, Shelley; Chen, Kaitlin; Sayal, Sachin; Prabahan, Gajeni; Rabey, Mary R; Le, Lisa W; Seto, Andrew; Shepherd, Frances A; Liu, Geoffrey; Bradbury, Penelope; Sacher, Adrian G; Law, Jennifer H; Sabatini, Peter; Stockley, Tracy L; Tsao, Ming S; Leighl, Natasha B.
Afiliación
  • Kuang S; Department of Medical Oncology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1M9, Canada.
  • Chen K; Department of Medical Oncology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1M9, Canada.
  • Sayal S; Department of Medical Oncology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1M9, Canada.
  • Prabahan G; Department of Medical Oncology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1M9, Canada.
  • Rabey MR; Department of Medical Oncology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1M9, Canada.
  • Le LW; Department of Biostatistics, University Health Network, Toronto, ON M5G 2C1, Canada.
  • Seto A; Division of Clinical Laboratory Genetics, Laboratory Medicine Program, University Health Network, Toronto, ON M5G 2C4, Canada.
  • Shepherd FA; Department of Medical Oncology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1M9, Canada.
  • Liu G; Department of Medical Oncology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1M9, Canada.
  • Bradbury P; Department of Medical Oncology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1M9, Canada.
  • Sacher AG; Department of Medical Oncology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1M9, Canada.
  • Law JH; Department of Medical Oncology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1M9, Canada.
  • Sabatini P; Department of Biostatistics, University Health Network, Toronto, ON M5G 2C1, Canada.
  • Stockley TL; Advanced Molecular Diagnostics Laboratory, University Health Network, Toronto, ON M5G 2M9, Canada.
  • Tsao MS; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Leighl NB; Department of Biostatistics, University Health Network, Toronto, ON M5G 2C1, Canada.
Curr Oncol ; 31(8): 4476-4485, 2024 Aug 03.
Article en En | MEDLINE | ID: mdl-39195317
ABSTRACT
In advanced non-squamous non-small-cell lung cancer (NSCLC), routine testing with next-generation sequencing (NGS) is recommended to identify actionable genomic alterations (AGAs). The therapeutic implications of repeated NGS testing on synchronous and metachronous tumors are unclear. Between February 2017 and October 2020, NSCLC samples from a single institution were reflex-tested using a targeted 15-gene NGS panel (TruSight Tumor 15, Illumina). Thirty-eight patients were identified with multiple NGS results from 82 samples 11% were from single unifocal, 51% were from synchronous, and 38% were from metachronous tumors. Changes in EGFR, KRAS, PI3KCA, and TP53 variants were found in 22 patients' samples (58%). No changes were seen with longitudinal testing of multiple samples from single unifocal tumors, while changes were observed in 60% of synchronous and 71% of metachronous tumors. Of these, 26% of patients had AGA differences between samples. Acknowledging the limited sample size, a significant difference in overall survival was observed between synchronous separate primaries and metastasis. Repeat NGS testing of synchronous and metachronous NSCLC tumors may identify differing variants in >50% of patients. These changes may reflect separate primary lung carcinomas, tumor heterogeneity among intrapulmonary metastases, and clonal evolution. NGS testing of multiple tumors may enhance the identification of therapeutic targets for treatment decisions.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Pulmón de Células no Pequeñas / Secuenciación de Nucleótidos de Alto Rendimiento / Neoplasias Pulmonares Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Curr Oncol Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinoma de Pulmón de Células no Pequeñas / Secuenciación de Nucleótidos de Alto Rendimiento / Neoplasias Pulmonares Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Curr Oncol Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza