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Salivary Proteins in Human Acquired Enamel Pellicle (AEP) on Eroded and Uneroded Teeth in Patients with Gastro-Oesophageal Reflux Disease (GORD).
Alharthi, Rasha; Mutahar, Mahdi; Bartlett, David; Jafari, Jafar; Moazzez, Rebecca.
Afiliación
  • Alharthi R; Department of Clinical Dental Sciences, College of Dentistry, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia.
  • Mutahar M; School of Dentistry, Health and Care Professions, University of Portsmouth, Portsmouth PO1 2QG, UK.
  • Bartlett D; Centre for Oral Clinical and Translational Sciences, King's College London, London SE1 9RT, UK.
  • Jafari J; Upper GI Physiology Service, Guy's and St. Thomas' Hospitals NHS Foundation Trust, London SE1 9RT, UK.
  • Moazzez R; Department of Preventive and Restorative Dentistry, University of the Pacific, San Francisco, CA 94103, USA.
Dent J (Basel) ; 12(8)2024 Jul 26.
Article en En | MEDLINE | ID: mdl-39195079
ABSTRACT
The aim of this in vivo study was to compare total protein present in the salivary films (F) and acquired enamel pellicle (AEP) on eroded and non-eroded surfaces in patients suffering from GORD symptoms with and without GORD diagnosis (GORD, No-GORD). Thirty-nine patients suffering from GORD symptoms and erosive tooth wear on lower first molars and an unaffected posterior occlusal surface in the same quadrant were recruited from Guy's hospital, London. Salivary film and AEP were collected from the eroded and uneroded occlusal surfaces, using 0.5% sodium dodecyl sulphate (SDS)-soaked filter papers. Total protein concentration was analysed using bicinchoninic acid assay (BCA). Statistical analysis was conducted using Shapiro-Wilk, ANOVA, and Tukey's tests (p < 0.05), comparing four GDS sample types and GORD vs. No-GORD groups. The level of significance was set as p < 0.05. Data were compared between eroded and uneroded surfaces in the same patient with GORD symptoms, as well as between those with or without a GORD diagnosis (GORD, No-GORD). The AEP total protein concentration from the eroded [2.17 (0.49) mg/mL] and uneroded surfaces [2.24 (0.66) mg/mL] of the GORD group were statistically significantly lower than those on eroded [3.27 (1.01) mg/mL] and uneroded [3.33 (1.57) mg/mL] surfaces in the No-GORD group (p = 0.007) (p = 0.008), respectively. No statistically significant differences were observed for film and AEP between eroded and uneroded surfaces (p > 0.05).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Dent J (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Dent J (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Suiza