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Identification of Secondary Metabolites by UHPLC-ESI-HRMS/MS in Antifungal Strain Trichoderma harzianum (LBAT-53).
Hernández, Giselle; Ponce de la Cal, Amaia; Louis, Yuset; Baró Robaina, Yamilé; Coll, Yamilet; Spengler, Iraida; Mirabal-Gallardo, Yaneris.
Afiliación
  • Hernández G; Center for Natural Products Research, Faculty of Chemistry, University of Havana, Havana 10400, Cuba.
  • Ponce de la Cal A; Plant Health Research Institute (INISAV), 110 Str. 514, Havana 11600, Cuba.
  • Louis Y; Center for Natural Products Research, Faculty of Chemistry, University of Havana, Havana 10400, Cuba.
  • Baró Robaina Y; Plant Health Research Institute (INISAV), 110 Str. 514, Havana 11600, Cuba.
  • Coll Y; Center for Natural Products Research, Faculty of Chemistry, University of Havana, Havana 10400, Cuba.
  • Spengler I; Center for Natural Products Research, Faculty of Chemistry, University of Havana, Havana 10400, Cuba.
  • Mirabal-Gallardo Y; Faculty of Engineering, Institute of Applied Chemistry, Universidad Autónoma de Chile, Talca 3460000, Chile.
J Fungi (Basel) ; 10(8)2024 Aug 03.
Article en En | MEDLINE | ID: mdl-39194873
ABSTRACT
Trichoderma spp. are filamentous fungi generally observed in nature, which are widely marketed as biocontrol agents. The secondary metabolites produced have obtained special attention since they possess attractive chemical structures with a broad spectrum of biological activities. In Cuba, the species of Trichoderma have been commercially applied for the control of several phytopathogens to protect agricultural crops, but few studies have been carried out to detect and characterize the production of metabolites with biological activity. The strain Trichoderma harzianum LBAT-53 was subjected to an antifungal in vitro assay against Fusarium oxysporum f.sp. cubense by dual culture and volatile metabolite assays and fermented in PDB under constant agitation conditions. The ethyl acetate crude extract was obtained by liquid-liquid extraction. The fungal extract was investigated for the composition of secondary metabolites through chemical screening and ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) in negative ionization mode. As a result, LBAT-53 showed antagonistic activity in vitro (Class 2) against the pathogen evaluated in direct confrontation (76.9% of inhibition in 10 days) and by volatile metabolites (<40% in 7 days). Furthermore, seven low-molecular-weight phenolic compounds, including chrysophanol, phomarin, endocrocin, and trichophenol A, among others, were identified using UHPLC-ESI-MS/MS. This study is the first work on the characterization of secondary metabolites produced by the commercially applied strain LBAT-53, which is a promising source of bioactive compounds. These results provide a better understanding of the metabolism of this fungus, which is widely used in Cuba as biopesticides in agriculture pest control.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Fungi (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Cuba Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Fungi (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Cuba Pais de publicación: Suiza