The Role of Mannitol and Vitamin D in Ovarian Ischemia/Reperfusion Injury in Rats with Acute Abdominal.

Karateke, Faruk; Karateke, Atilla; Topdagi, Basak; Atilgan, Merve; Dokuyucu, Recep

Karateke, Faruk; Karateke, Atilla; Topdagi, Basak; Atilgan, Merve; Dokuyucu, Recep.

Afiliación

Karateke F; Department of General Surgery, Adana Private Middle East Hospital, 01140 Adana, Turkey.
Karateke A; Department of Gynecology and Obstetrics, Private Reyhanli MMT Amerikan Hospital, 31500 Hatay, Turkey.
Topdagi B; Department of Dentistry, School of Medicine, Sultan II. Abdulhamid Han Training and Research Hospital, 34668 Istanbul, Turkey.
Atilgan M; Department of Pediatric Surgery, School of Medicine, Necmettin Erbakan University, 42090 Konya, Turkey.
Dokuyucu R; Department of Physiology, Medical Specialization Training Center (TUSMER), 06420 Ankara, Turkey.

Curr Issues Mol Biol ; 46(8): 8903-8913, 2024 Aug 15.

Article en En | MEDLINE | ID: mdl-39194743

ABSTRACT

ABSTRACT

This study was designed to investigate the effects of vitamin D and mannitol in an experimental rat ovarian torsion model. Thirty-two female Wistar albino rats were randomly classified as group 1 (sham), group 2 (detorsion), group 3 (detorsion + mannitol), group 4 (detorsion + vitamin D) and group 5 (detorsion + mannitol + vitamin D) (for each group n = 8). All groups were subjected to bilateral adnexal torsion for 2 h except for group 1. Bilateral adnexal detorsion was performed in all groups except for group 1. Groups 3 and 5 intraperitoneally received the injection of mannitol at a dose of 0.3 mg/kg 30 min before detorsion. Also, the group's 4 and 5 orally received vitamin D in a dose of 500 IU/kg/day for two weeks before torsion. Total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI) and proliferating cell nuclear antigen (PCNA) levels were analyzed. According to the histopathological analyses, ovarian tissue damage and follicle counting were evaluated. TOS, OSI and histopathologic score values of ovarian tissue were significantly lower in group 5 than groups 2, 3 and 4 (p < 0.05). The PCNA level was significantly higher in group 5 than in groups 2, 3 and 4 (p < 0.05). A strong negative correlation was found between OSI and PCNA in groups 2, 3, 4 and 5 (r = -0.92, p = 0.01; r = -0.98, p < 0.0001; r = -0.98, p < 0.0001 and r = -0.96, p = 0.0002, respectively). The numbers of primordial follicles in group 5 (p < 0.001) and primary follicles in group 4 (p < 0.001) were significantly higher when compared to group 2. Based on the results of this study, it could be suggested that combination treatment of mannitol with vitamin D is more effective in reversing tissue damage induced by ischemia-reperfusion (I/R) injury in the ovarian torsion model than administration of only an agent.

Palabras clave

oxidative stress index (OSI); proliferating cell nuclear antigen (PCNA); total antioxidant status (TAS); total oxidant status (TOS)

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Curr Issues Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Suiza

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