[Thiotert Induces Myelodysplastic Syndromes Cells Apoptosis by Activating Oxidative Stress].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 32(4): 1181-1185, 2024 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-39192416
ABSTRACT
OBJECTIVE:
To explore whether thiotert treatment can inhibit proliferation and induce apoptosis in myelodysplastic syndromes (MDS) cells.METHODS:
CCK-8 assay was used for determining the cytotoxicity of thiotert to MDS cell line SKM-1 and the reversal effect of GSH, NAC, and Z-VAD-FMK on thiotert-induced inhibition of cell viability. EdU assay was deployed to detect the cell proliferation ability. Intracellular reactive oxygen species (ROS) was measured by flow cytometry after DCFH-DA staining. The expression of DNA damage- and apoptosis-related proteins was detected by Western blot.RESULTS:
Thiotert treatment significantly suppressed the cell viability and proliferation ability in SKM-1 cells. A large amount of ROS generation and markedly elevated C-PARP, C-Caspase 3, and γ-H2AX were observed after thiotert administration, while BCL-2 was significantly decreased. In addition, GSH, NAC, and Z-VAD-FMK were able to mitigate the cytotoxicity of thiotert on SKM-1 cells.CONCLUSION:
Thiotert can promote MDS cell apoptosis by mediating ROS production and pro-apoptotic proteins expression.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Síndromes Mielodisplásicos
/
Especies Reactivas de Oxígeno
/
Apoptosis
/
Estrés Oxidativo
/
Proliferación Celular
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Asunto de la revista:
HEMATOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China