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Retrospective ANalysis of multi-drug resistant Gram-nEgative bacteRia on veno-venous extracorporeal membrane oxygenation. The multicenter RANGER STUDY.
Boscolo, Annalisa; Bruni, Andrea; Giani, Marco; Garofalo, Eugenio; Sella, Nicolò; Pettenuzzo, Tommaso; Bombino, Michela; Palcani, Matteo; Rezoagli, Emanuele; Pozzi, Matteo; Falcioni, Elena; Pistollato, Elisa; Biamonte, Eugenio; Murgolo, Francesco; D'Arrigo, Graziella; Gori, Mercedes; Tripepi, Giovanni Luigi; Gottin, Leonardo; Longhini, Federico; Grasso, Salvatore; Navalesi, Paolo; Foti, Giuseppe.
Afiliación
  • Boscolo A; Department of Medicine (DIMED), University of Padua, 13 Gallucci Street, 35121, Padua, Italy.
  • Bruni A; Institute of Anesthesia and Critical Care, Padua University Hospital, Padua, Italy.
  • Giani M; Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padua, Padua, Italy.
  • Garofalo E; Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy.
  • Sella N; School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy.
  • Pettenuzzo T; Department of Emergency and Critical Care, IRCSS San Gerardo Dei Tintori, Monza, Italy.
  • Bombino M; Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy.
  • Palcani M; Institute of Anesthesia and Critical Care, Padua University Hospital, Padua, Italy.
  • Rezoagli E; Institute of Anesthesia and Critical Care, Padua University Hospital, Padua, Italy.
  • Pozzi M; Department of Emergency and Critical Care, IRCSS San Gerardo Dei Tintori, Monza, Italy.
  • Falcioni E; School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy.
  • Pistollato E; School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy.
  • Biamonte E; Department of Emergency and Critical Care, IRCSS San Gerardo Dei Tintori, Monza, Italy.
  • Murgolo F; School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy.
  • D'Arrigo G; Department of Emergency and Critical Care, IRCSS San Gerardo Dei Tintori, Monza, Italy.
  • Gori M; Department of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, Verona, Italy.
  • Tripepi GL; Cardiothoracic and Vascular Intensive Care Unit, Verona University Hospital, Verona, Italy.
  • Gottin L; Department of Medicine (DIMED), University of Padua, 13 Gallucci Street, 35121, Padua, Italy.
  • Longhini F; Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy.
  • Grasso S; Department of Precision and Regenerative Medicine and Ionian Area, School of Medicine, University of Bari "Aldo Moro", Bari, Italy.
  • Navalesi P; CNR-IFC, Institute of Clinical Physiology of Reggio Calabria, Reggio Calabria, Italy.
  • Foti G; CNR-IFC, Institute of Clinical Physiology of Rome, Rome, Italy.
Crit Care ; 28(1): 279, 2024 Aug 27.
Article en En | MEDLINE | ID: mdl-39192287
ABSTRACT

BACKGROUND:

Veno-venous extracorporeal membrane oxygenation (V-V ECMO) is a rapidly expanding life-support technique worldwide. The most common indications are severe hypoxemia and/or hypercapnia, unresponsive to conventional treatments, primarily in cases of acute respiratory distress syndrome. Concerning potential contraindications, there is no mention of microbiological history, especially related to multi-drug resistant (MDR) bacteria isolated before V-V ECMO placement. Our study aims to investigate (i) the prevalence and incidence of MDR Gram-negative (GN) bacteria in a cohort of V-V ECMOs; (ii) the risk of 1-year mortality, especially in the case of predetected MDR GN bacteria; and (iii) the impact of annual hospital V-V ECMO volume on the probability of acquiring MDR GN bacteria.

METHODS:

All consecutive adults admitted to the Intensive Care Units of 5 Italian university-affiliated hospitals and requiring V-V ECMO were screened. Exclusion criteria were age < 18 years, pregnancy, veno-arterial or mixed ECMO-configuration, incomplete records, survival < 24 h after V-V ECMO. A standard protocol of microbiological surveillance was applied and MDR profiles were identified using in vitro susceptibility tests. Cox-proportional hazards models were applied for investigating mortality.

RESULTS:

Two hundred and seventy-nine V-V ECMO patients (72% male) were enrolled. The overall MDR GN bacteria percentage was 50% 21% (n.59) detected before and 29% (n.80) after V-V ECMO placement. The overall 1-year mortality was 42%, with a higher risk observed in predetected patients (aHR 2.14 [1.33-3.47], p value 0.002), while not in 'V-V ECMO-acquired MDR GN bacteria' group (aHR 1.51 [0.94-2.42], p value 0.090), as compared to 'non-MDR GN bacteria' group (reference). Same findings were found considering only infections. A larger annual hospital V-V ECMO volume was associated with a lower probability of acquiring MDR GN bacteria during V-V ECMO course (aOR 0.91 [0.86-0.97], p value 0.002).

CONCLUSIONS:

21% of MDR GN bacteria were detected before; while 29% after V-V ECMO connection. A history of MDR GN bacteria, isolated before V-V ECMO, was an independent risk factor for mortality. The annual hospital V-V ECMO volume affected the probability of acquiring MDR GN bacteria. Trial Registration ClinicalTrial.gov Registration Number NCTNCT06199141, date 12.26.2023.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxigenación por Membrana Extracorpórea / Farmacorresistencia Bacteriana Múltiple / Bacterias Gramnegativas Límite: Adult / Aged / Female / Humans / Male / Middle aged País/Región como asunto: Europa Idioma: En Revista: Crit Care Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxigenación por Membrana Extracorpórea / Farmacorresistencia Bacteriana Múltiple / Bacterias Gramnegativas Límite: Adult / Aged / Female / Humans / Male / Middle aged País/Región como asunto: Europa Idioma: En Revista: Crit Care Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido