Phagosome Isolation with Magnetic Beads and Purification of Toll-Like Receptor (TLR) Complexes in Phagosomes.

Liu, Wei; Liu, Bo

Liu, Wei; Liu, Bo.

Afiliación

Liu W; Key Laboratory of Immune Response and Immunotherapy, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China.
Liu B; Key Laboratory of Immune Response and Immunotherapy, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China. bliu@ips.ac.cn.

Methods Mol Biol ; 2854: 171-175, 2025.

Article en En | MEDLINE | ID: mdl-39192128

ABSTRACT

ABSTRACT

Phagocytosis is a central process by which macrophage cells internalize and eliminate microbes as well as apoptotic cells. The nascent phagosome undergoes a complex maturation process involving sequential fusion with endosomal compartments. The endosomal TLRs, including TLR3, -7, -8, and -9, play a critical role in innate immunity by sensing bacterial or viral nucleic acids and are preferentially transported to the phagosomal membrane of innate immune cells upon activation. Therefore, phagosome isolation is helpful for studies on pathogenic invasion and the functions of phagosome proteins, including endosomal TLRs.

Asunto(s)

Fagosomas; Receptores Toll-Like; Fagosomas/metabolismo; Receptores Toll-Like/metabolismo; Animales; Fagocitosis; Ratones; Humanos; Inmunidad Innata; Macrófagos/metabolismo; Macrófagos/inmunología

Palabras clave

Endosomal TLR; Organelle purification; Phagosome

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fagosomas / Receptores Toll-Like Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2025 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

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