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Identifying a Ubiquitinated Adaptor Protein by a Viral E3 Ligase Through Co-immunoprecipitation.
Su, Chenhe; Su, Chenhao; Zheng, Chunfu.
Afiliación
  • Su C; State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, NMPA Key Laboratory for Research and Evaluation of Innovative Drug, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan, China.
  • Su C; Department of Nephrology and Rheumatology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
  • Zheng C; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol ; 2854: 35-40, 2025.
Article en En | MEDLINE | ID: mdl-39192116
ABSTRACT
Co-immunoprecipitation is a technique widely utilized to isolate protein complexes and study protein-protein interactions. Ubiquitinated proteins could be identified by combining co-immunoprecipitation with SDS-PAGE followed by immunoblotting. In this chapter, we use Herpes Simplex Virus 1 immediate-early protein ICP0-mediated polyubiquitination of p50 as an example to describe the method to identify a ubiquitinated adaptor protein by a viral E3 ligase by co-immunoprecipitation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Inmediatas-Precoces / Ubiquitina-Proteína Ligasas / Inmunoprecipitación / Ubiquitinación Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2025 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Inmediatas-Precoces / Ubiquitina-Proteína Ligasas / Inmunoprecipitación / Ubiquitinación Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2025 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos