Identifying an Abnormal Phosphorylated Adaptor by Viral Kinase Using Mass Spectrometry.
Methods Mol Biol
; 2854: 29-34, 2025.
Article
en En
| MEDLINE
| ID: mdl-39192115
ABSTRACT
Mass spectrometers are widely used to identify protein phosphorylation sites. The process usually involves selective isolation of phosphoproteins and subsequent fragmentation to identify both the peptide sequence and phosphorylation site. Immunoprecipitation could capture and purify the protein of interest, greatly reducing sample complexity before submitting it for mass spectrometry analysis. This chapter describes a method to identify an abnormal phosphorylated site of the adaptor protein by a viral kinase through immunoprecipitation followed by LC-MS/MS.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfoproteínas
/
Inmunoprecipitación
/
Espectrometría de Masas en Tándem
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2025
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Estados Unidos