Effects and mechanisms of chlormequat on horizontal transfer of antibiotic resistance genes through plasmid-mediated conjugation in agro-ecosystems.

Zhao, Hui; Sun, Yulong; Cao, Xi; Waigi, Michael Gatheru; Liu, Juan

Zhao, Hui; Sun, Yulong; Cao, Xi; Waigi, Michael Gatheru; Liu, Juan.

Afiliación

Zhao H; Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Sun Y; Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Cao X; Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Waigi MG; Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Liu J; Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: liujuan@njau.edu.cn.

J Hazard Mater ; 479: 135639, 2024 Nov 05.

Article en En | MEDLINE | ID: mdl-39191006

ABSTRACT

ABSTRACT

Chlormequat (CCC) is widely used in agricultural production to increase the crop yield. However, the effects of CCC on transfer of ARGs in agricultural system are still unclear. In this study, using E.coli DH5α (carrying RP4 plasmid with AmpR, TetR, KanR) as the donor bacterium, E.coli HB101, endophytic Pseudomonas sp. Ph6 or rhizosphere Pseudomonas putida KT2440 as the recipient strain, three conjugative systems were designed to investigate the effects of CCC on ARG transfer. Meanwhile, hydroponics experiments were designed to study the ARG spread in the rice-nutrient solution system after CCC application. The results showed that CCC significantly promoted the RP4 conjugation by expanding cell membrane permeability and improving the relative transcription levels of trfAp, trbBp, traA and traL genes in RP4. Furthermore, the conjugation frequency between E. coli and Pseudomonas was much higher than that between E. coli cells. Compared with spraying foliage with 2500 mg·L-1 of CCC, soaking seeds with 250 mg·L-1 of CCC was more beneficial to the colonization of ARB in rice, and also increased the abundance of ARGs in rice cultivation system. These results remind that the use of CCC in agricultural production might promote the ARG transmission in agro-ecosystems; however, foliage spraying with 2500 mg·L-1 of CCC could control its spread.

Asunto(s)

Conjugación Genética; Escherichia coli; Transferencia de Gen Horizontal; Oryza; Plásmidos; Pseudomonas; Plásmidos/genética; Oryza/microbiología; Oryza/crecimiento & desarrollo; Oryza/efectos de los fármacos; Oryza/genética; Escherichia coli/genética; Escherichia coli/efectos de los fármacos; Pseudomonas/genética; Pseudomonas/efectos de los fármacos; Pseudomonas/metabolismo; Pseudomonas putida/genética; Pseudomonas putida/efectos de los fármacos; Pseudomonas putida/metabolismo; Genes Bacterianos/efectos de los fármacos; Antibacterianos/farmacología; Agricultura; Farmacorresistencia Bacteriana/genética; Farmacorresistencia Bacteriana/efectos de los fármacos; Farmacorresistencia Microbiana/genética

Palabras clave

Antibiotic resistance genes; Chlormequat; Dioparent conjugation; Regulatory genes; Seed soaking

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásmidos / Pseudomonas / Oryza / Conjugación Genética / Transferencia de Gen Horizontal / Escherichia coli Idioma: En Revista: J Hazard Mater Asunto de la revista: SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos

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