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Imprinted X chromosome inactivation in marsupials: The paternal X arrives at the egg with a silent DNA methylation profile.
Milton, Ashley M; Marín-Gual, Laia; Lister, Nicholas C; McIntyre, Kim L; Grady, Patrick G S; Laird, Melanie K; Bond, Donna M; Hore, Timothy A; O'Neill, Rachel J; Pask, Andrew J; Renfree, Marilyn B; Ruiz-Herrera, Aurora; Waters, Paul D.
Afiliación
  • Milton AM; School of Biotechnology and Biomolecular Sciences, Faculty of Science, The University of New South Wales, Sydney, NSW 2052, Australia.
  • Marín-Gual L; Departament de Biologia Cellular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Spain.
  • Lister NC; Genome Integrity and Instability Group, Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Spain.
  • McIntyre KL; School of Biotechnology and Biomolecular Sciences, Faculty of Science, The University of New South Wales, Sydney, NSW 2052, Australia.
  • Grady PGS; School of Biotechnology and Biomolecular Sciences, Faculty of Science, The University of New South Wales, Sydney, NSW 2052, Australia.
  • Laird MK; Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269.
  • Bond DM; Institute for Systems Genomics, University of Connecticut, Storrs, CT 06269.
  • Hore TA; Department of Anatomy, University of Otago, Dunedin 9016, New Zealand.
  • O'Neill RJ; Department of Anatomy, University of Otago, Dunedin 9016, New Zealand.
  • Pask AJ; Department of Anatomy, University of Otago, Dunedin 9016, New Zealand.
  • Renfree MB; Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269.
  • Ruiz-Herrera A; Institute for Systems Genomics, University of Connecticut, Storrs, CT 06269.
  • Waters PD; School of BioSciences, The University of Melbourne, Parkville, VIC 3010, Australia.
Proc Natl Acad Sci U S A ; 121(36): e2412185121, 2024 Sep 03.
Article en En | MEDLINE | ID: mdl-39190362
ABSTRACT
X chromosome inactivation (XCI) is an epigenetic process that results in the transcriptional silencing of one X chromosome in the somatic cells of females. This phenomenon is common to both eutherian and marsupial mammals, but there are fundamental differences. In eutherians, the X chosen for silencing is random. DNA methylation on the eutherian inactive X is high at transcription start sites (TSSs) and their flanking regions, resulting in universally high DNA methylation. This contrasts XCI in marsupials where the paternally derived X is always silenced, and in which DNA methylation is low at TSSs and flanking regions. Here, we examined the DNA methylation status of the tammar wallaby X chromosome during spermatogenesis to determine the DNA methylation profile of the paternal X prior to and at fertilization. Whole genome enzymatic methylation sequencing was carried out on enriched flow-sorted populations of premeiotic, meiotic, and postmeiotic cells. We observed that the X displayed a pattern of DNA methylation from spermatogonia to mature sperm that reflected the inactive X in female somatic tissue. Therefore, the paternal X chromosome arrives at the egg with a DNA methylation profile that reflects the transcriptionally silent X in adult female somatic tissue. We present this epigenetic signature as a candidate for the long sought-after imprint for paternal XCI in marsupials.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosoma X / Metilación de ADN / Inactivación del Cromosoma X Límite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosoma X / Metilación de ADN / Inactivación del Cromosoma X Límite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos