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Bacterial amyloid curli activates the host unfolded protein response via IRE1α in the presence of HLA-B27.
Grando, Kaitlyn; Bessho, Shingo; Harrell, Kayla; Kyrylchuk, Kathrine; Pantoja, Alejandro M; Olubajo, Sophia; Albicoro, Francisco J; Klein-Szanto, Andres; Tükel, Çagla.
Afiliación
  • Grando K; Center for Microbiology and Immunology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
  • Bessho S; Center for Microbiology and Immunology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
  • Harrell K; Center for Microbiology and Immunology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
  • Kyrylchuk K; Center for Microbiology and Immunology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
  • Pantoja AM; Center for Microbiology and Immunology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
  • Olubajo S; Center for Microbiology and Immunology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
  • Albicoro FJ; Center for Microbiology and Immunology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
  • Klein-Szanto A; Histopathology Facility, Fox Chase Cancer Center, Philadelphia, PA, USA.
  • Tükel Ç; Center for Microbiology and Immunology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
Gut Microbes ; 16(1): 2392877, 2024.
Article en En | MEDLINE | ID: mdl-39189642
ABSTRACT
Salmonella enterica serovar Typhimurium (STm) causes gastroenteritis and can progress to reactive arthritis (ReA). STm forms biofilms in the gut that secrete the amyloid curli, which we previously demonstrated can trigger autoimmunity in mice. HLA-B27 is a genetic risk factor for ReA; activation of the unfolded protein response (UPR) due to HLA-B27 misfolding is thought to play a critical role in ReA pathogenesis. To determine whether curli exacerbates HLA-B27-induced UPR, bone marrow-derived macrophages (BMDMs) isolated from HLA-B27 transgenic (tg) mice were used. BMDMs treated with purified curli exhibited elevated UPR compared to C57BL/6, and curli-induced IL-6 was reduced by pre-treating macrophages with inhibitors of the IRE1α branch of the UPR. In BMDMs, intracellular curli colocalized with GRP78, a regulator of the UPR. In vivo, acute infection with wild-type STm increased UPR markers in the ceca of HLA-B27tg mice compared to C57BL/6. STm biofilms that contain curli were visible in the lumen of cecal tissue sections. Furthermore, curli was associated with macrophages in the lamina propria, colocalizing with GRP78. Together, these results suggest that UPR plays a role in the curli-induced inflammatory response, especially in the presence of HLA-B27, a possible mechanistic link between STm infection and genetic susceptibility to ReA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella typhimurium / Proteínas Bacterianas / Antígeno HLA-B27 / Proteínas Serina-Treonina Quinasas / Endorribonucleasas / Respuesta de Proteína Desplegada / Chaperón BiP del Retículo Endoplásmico / Macrófagos Límite: Animals / Humans Idioma: En Revista: Gut Microbes Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella typhimurium / Proteínas Bacterianas / Antígeno HLA-B27 / Proteínas Serina-Treonina Quinasas / Endorribonucleasas / Respuesta de Proteína Desplegada / Chaperón BiP del Retículo Endoplásmico / Macrófagos Límite: Animals / Humans Idioma: En Revista: Gut Microbes Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos