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Photobiomodulation Effect of Diode Laser on Differentiation of Osteoprogenitor Cells in Rat Bone Marrow.
Iso, Eisuke; Yamazaki, Takahide; Kobayashi, Norika; Kadokura, Hiroshi; Tsuchiya, Takako; Kato, Yuka; Yokose, Satoshi.
Afiliación
  • Iso E; Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Sakado, Japan.
  • Yamazaki T; Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Sakado, Japan.
  • Kobayashi N; Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Sakado, Japan.
  • Kadokura H; Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Sakado, Japan.
  • Tsuchiya T; Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Sakado, Japan.
  • Kato Y; Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Sakado, Japan.
  • Yokose S; Division of Endodontics and Operative Dentistry, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Sakado, Japan s-yokose@dent.meikai.ac.jp.
In Vivo ; 38(5): 2214-2219, 2024.
Article en En | MEDLINE | ID: mdl-39187344
ABSTRACT
BACKGROUND/

AIM:

Bone marrow cells contain nonhematopoietic cells with the ability to differentiate into osteogenic, chondrogenic, and adipogenic lineages. Mechanical stress influences osteoblast differentiation of bone marrow cells into osteogenic, chondrogenic, and adipogenic lineages, measurable as the abundance of alkaline phosphatase-positive (ALP+) colony-forming unit-fibroblasts (CFU-F); however, the effect of diode laser irradiation on osteoblast differentiation is unknown. The aim of this study was to analyze the effects of photobiomodulation on the osteogenic differentiation of mesenchymal stem cells in the bone marrow, using the CFU-F assay. MATERIALS AND

METHODS:

Bone marrow cells isolated from rat tibiae were cultured and irradiated with a diode laser (wavelength 808 nm) at a total energy of 0 J (control), 50 J, and 150 J.

RESULTS:

On day 7 after irradiation, ALP+ CFU-F were most abundant in the 50 J group and the least abundant in the 150 J group. Mineralized nodule formation was observed after long-term culture (21 days). Compared with the control group, there were significantly more nodules in the 50 J group and significantly fewer nodules in the 150 J group. Osteocalcin mRNA expression was highest in the 50 J group, and there was no difference between the control and 150 J groups.

CONCLUSION:

Irradiation with 50 J was effective in stimulating osteogenesis in bone marrow stem cells. These findings suggest that diode laser irradiation can induce osteogenesis in rat bone marrow cells in an energy-dependent manner, and appears suitable for application in bone regeneration therapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Osteogénesis / Células de la Médula Ósea / Diferenciación Celular / Láseres de Semiconductores / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: In Vivo Asunto de la revista: NEOPLASIAS Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Grecia
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Osteogénesis / Células de la Médula Ósea / Diferenciación Celular / Láseres de Semiconductores / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: In Vivo Asunto de la revista: NEOPLASIAS Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Grecia