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A novel method for the simultaneous determination of drugs of abuse, ethyl glucuronide and synthetic opioids in human hair through a single digestion, purification and analysis in LC-MS/MS.
Vandelli, D; Palazzoli, F; Verri, P; Castagnetti, V; Profeta, C; Borghi, A; Cecchi, R.
Afiliación
  • Vandelli D; Department of Biomedical, Metabolic and Neural Sciences, Institute of Legal Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy. Electronic address: daniele.vandelli@unimore.it.
  • Palazzoli F; Department of Biomedical, Metabolic and Neural Sciences, Institute of Legal Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy.
  • Verri P; Department of Biomedical, Metabolic and Neural Sciences, Institute of Legal Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy.
  • Castagnetti V; Department of Biomedical, Metabolic and Neural Sciences, Institute of Legal Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy.
  • Profeta C; Department of Biomedical, Metabolic and Neural Sciences, Institute of Legal Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy.
  • Borghi A; Department of Biomedical, Metabolic and Neural Sciences, Institute of Legal Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy.
  • Cecchi R; Department of Biomedical, Metabolic and Neural Sciences, Institute of Legal Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1246: 124269, 2024 Oct 01.
Article en En | MEDLINE | ID: mdl-39186886
ABSTRACT
Polydrug use is a serious health and social problem worldwide. Over the past several years, there has been an increasing tendency to combine narcotics, alcohol, sedatives, and/or stimulants. To the traditional drugs of abuse and alcohol, an increase of new abuse drugs such as synthetic opioids has been added. In the current study, the development and validation of an innovative and fast analytical procedure has been presented to determine drugs of abuse, ethyl glucuronide and synthetics opioids in 30 mg of human hair through a single digestion, purification and analysis in LC-MS/MS. A combine simple preparation of hair sample followed to a single chromatographic run of 10 min has been proposed. A full validation for 54 target analytes for the parameters of selectivity, linearity, limit of detection, limit of quantification, accuracy, precision, matrix effects, recovery, and dilution integrity was successful completed. The method was linear in different ranges with r values of at least 0.990; the value to the validated LLOQ values were in the range 0.1-100 pg/mg. The method offered satisfactory precisions (CV<15 % and accuracy ± 20 %). In conclusion, a significant reduction in the overall times of the analytical procedure and the reduction of consumables costs make this method extremely advantageous and undoubtedly useful in routine laboratory workflow analyses and open the way to the prospect of a further implementation which also includes other classes of xenobiotics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Drogas Ilícitas / Detección de Abuso de Sustancias / Espectrometría de Masas en Tándem / Límite de Detección / Glucuronatos / Cabello / Analgésicos Opioides Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Drogas Ilícitas / Detección de Abuso de Sustancias / Espectrometría de Masas en Tándem / Límite de Detección / Glucuronatos / Cabello / Analgésicos Opioides Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos