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Commensal-derived short-chain fatty acids disrupt lipid membrane homeostasis in Staphylococcus aureus.
Fletcher, Joshua R; Hansen, Lisa A; Martinez, Richard; Freeman, Christian D; Thorns, Niall; Villareal, Alex R; Penningroth, Mitchell R; Vogt, Grace A; Tyler, Matthew; Hines, Kelly M; Hunter, Ryan C.
Afiliación
  • Fletcher JR; Department of Microbiology & Immunology, University of Minnesota, Minneapolis, MN 55455.
  • Hansen LA; Department of Population Health and Pathobiology, North Carolina State University College of Veterinary Medicine, Raleigh, NC 27695.
  • Martinez R; Department of Microbiology & Immunology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, 14203.
  • Freeman CD; Department of Microbiology & Immunology, University of Minnesota, Minneapolis, MN 55455.
  • Thorns N; Department of Chemistry, University of Georgia, Athens, GA, 30602.
  • Villareal AR; Department of Microbiology & Immunology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, 14203.
  • Penningroth MR; Department of Microbiology & Immunology, University of Minnesota, Minneapolis, MN 55455.
  • Vogt GA; Department of Microbiology & Immunology, University of Minnesota, Minneapolis, MN 55455.
  • Tyler M; Department of Microbiology & Immunology, University of Minnesota, Minneapolis, MN 55455.
  • Hines KM; Department of Otolaryngology, University of Minnesota, Minneapolis, MN, 55455.
  • Hunter RC; Department of Chemistry, University of Georgia, Athens, GA, 30602.
bioRxiv ; 2024 Aug 12.
Article en En | MEDLINE | ID: mdl-39185181
ABSTRACT
The role of commensal anaerobic bacteria in chronic respiratory infections is unclear, yet they can exist in abundances comparable to canonical pathogens in vivo. Their contributions to the metabolic landscape of the host environment may influence pathogen behavior by competing for nutrients and creating inhospitable conditions via toxic metabolites. Here, we reveal a mechanism by which the anaerobe-derived short chain fatty acids (SCFAs) propionate and butyrate negatively affect Staphylococcus aureus physiology by disrupting branched chain fatty acid (BCFA) metabolism. In turn, BCFA impairment results in impaired growth, diminished expression of the agr quorum sensing system, as well as increased sensitivity to membrane-targeting antimicrobials. Altered BCFA metabolism also reduces S. aureus fitness in competition with Pseudomonas aeruginosa, suggesting that airway microbiome composition and the metabolites they produce and exchange directly impact pathogen succession over time. The pleiotropic effects of these SCFAs on S. aureus fitness and their ubiquity as metabolites in animals also suggests that they may be effective as sensitizers to traditional antimicrobial agents when used in combination.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos