Polyethylene glycol as an improved barrier to prevent fleeing in C. elegans.
MicroPubl Biol
; 20242024.
Article
en En
| MEDLINE
| ID: mdl-39185016
ABSTRACT
Caenorhabditis elegans studies can be constrained by worms escaping standard solid nematode growth medium (NGM) plates. When worms are in search of food or are avoiding pathogens, chemicals, and environmental stressors, they often exhibit a behavior known as "fleeing". Palmitic acid (PA) is sometimes used as a barrier "fence" to reduce fleeing under limited food and oxygen conditions. Here, we evaluate the efficacy of palmitic acid, polyethylene glycol (PEG) and copper as potential barriers to reduce fleeing under various environmental conditions. Our results indicate that PA and PEG each reduce fasted flee rate and do not obviously alter overall health and lifespan of the worms, while copper blunts worm growth and development. We also find that PEG is a more optimal tool than PA since it is more effective in fasted conditions, reduces flee rate in a pathogenic environment, and does not alter worm size.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
MicroPubl Biol
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos