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A GhLac1-centered transcriptional regulatory cascade mediates cotton resistance to Verticillium dahliae through the lignin biosynthesis pathway.
Xiao, Shenghua; Ming, Yuqing; Zhou, Shaoli; Dong, Xianman; Liu, Shiming; Zhang, Xiaojun; Zhang, Xianlong; Hu, Qin; Zhu, Longfu.
Afiliación
  • Xiao S; State Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, College of Agriculture, Guangxi University, Nanning 530005, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430000, Hubei, China. Electronic address: shxiao@gx
  • Ming Y; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430000, Hubei, China; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430000, Hubei, China.
  • Zhou S; State Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, College of Agriculture, Guangxi University, Nanning 530005, China.
  • Dong X; State Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, College of Agriculture, Guangxi University, Nanning 530005, China.
  • Liu S; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430000, Hubei, China; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430000, Hubei, China.
  • Zhang X; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430000, Hubei, China; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430000, Hubei, China.
  • Zhang X; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430000, Hubei, China; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430000, Hubei, China.
  • Hu Q; State Key Lab for Conservation and Utilization of Subtropical Agri-Biological Resources, College of Agriculture, Guangxi University, Nanning 530005, China; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430000, Hubei, China. Electronic address: huqin-050
  • Zhu L; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430000, Hubei, China; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430000, Hubei, China. Electronic address: lfzhu@mail.hzau.edu.cn.
Int J Biol Macromol ; 279(Pt 1): 135042, 2024 Nov.
Article en En | MEDLINE | ID: mdl-39182876
ABSTRACT
The lignin biosynthesis pathway plays a crucial role in the defense response against V. dahliae in cotton, and it is essential to identify the key regulators in this pathway for disease-resistant breeding. In a previous study, the cotton laccase gene GhLac1 was identified as mediating plant broad-spectrum biotic stress tolerance by manipulating phenylpropanoid metabolism. However, the upstream master regulators and regulatory mechanism of lignin are still largely unknown. This study aims to identify the upstream regulators of GhLac1 and explore the molecular mechanism underlying cotton's disease resistance response to V. dahliae. Through the study, three WRKY, three MYB, and one APETALA2/ETHYLENE RESPONSIVE FACTOR (ERF) TFs were identified as differentially responding to V. dahliae infection in cotton. Among these TFs, GhWRKY30, GhWRKY41, GhMYB42, and GhTINY2 were found to directly bind to the GhLac1 promoter and activate its expression. Transient overexpression of these four TFs in cotton led to increased expression of GhLac1 and other the laccase family members, while knockdown of these TFs resulted in reduced lignin accumulation and increased susceptibility to V. dahliae. Additionally, GhWRKY30 and GhWRKY41 were observed to interact with themselves and with each other, synergistically transactivating the GhLac1 promoter. This study reveals a GhLac1-centered transcriptional regulatory cascade of lignin synthesis that contributes to cotton's defense response by modulating lignin metabolism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Proteínas de Plantas / Factores de Transcripción / Regulación de la Expresión Génica de las Plantas / Gossypium / Resistencia a la Enfermedad / Lignina Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Proteínas de Plantas / Factores de Transcripción / Regulación de la Expresión Génica de las Plantas / Gossypium / Resistencia a la Enfermedad / Lignina Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos