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IMPRESS: Improved methylation profiling using restriction enzymes and smMIP sequencing, combined with a new biomarker panel, creating a multi-cancer detection assay.
Vandenhoeck, Janah; Neefs, Isabelle; Vanpoucke, Thomas; Ibrahim, Joe; Suls, Arvid; Peeters, Dieter; Schepers, Anne; Hoischen, Alexander; Fransen, Erik; Peeters, Marc; Van Camp, Guy; Op de Beeck, Ken.
Afiliación
  • Vandenhoeck J; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.
  • Neefs I; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium.
  • Vanpoucke T; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.
  • Ibrahim J; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium.
  • Suls A; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.
  • Peeters D; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium.
  • Schepers A; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.
  • Hoischen A; Centre for Oncological Research Antwerp (CORE), University of Antwerp and Antwerp University Hospital, Wilrijk, Belgium.
  • Fransen E; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.
  • Peeters M; Department of Pathology, Antwerp University Hospital and University of Antwerp, Edegem, Belgium.
  • Van Camp G; Centre of Medical Genetics, University of Antwerp and Antwerp University Hospital, Edegem, Belgium.
  • Op de Beeck K; Department of Human Genetics and Radboud Institute for Molecular Life Sciences, Radboud University Medical Centre, Nijmegen, the Netherlands.
Br J Cancer ; 131(7): 1224-1236, 2024 Oct.
Article en En | MEDLINE | ID: mdl-39181941
ABSTRACT

BACKGROUND:

Despite the worldwide progress in cancer diagnostics, more sensitive diagnostic biomarkers are needed. The methylome has been extensively investigated in the last decades, but a low-cost, bisulfite-free detection method for multiplex analysis is still lacking.

METHODS:

We developed a methylation detection technique called IMPRESS, which combines methylation-sensitive restriction enzymes and single-molecule Molecular Inversion Probes. We used this technique for the development of a multi-cancer detection assay for eight of the most lethal cancer types worldwide. We selected 1791 CpG sites that can distinguish tumor from normal tissue based on DNA methylation. These sites were analysed with IMPRESS in 35 blood, 111 tumor and 114 normal samples. Finally, a classifier model was built.

RESULTS:

We present the successful development of IMPRESS and validated it with ddPCR. The final classifier model discriminating tumor from normal samples was built with 358 CpG target sites and reached a sensitivity of 0.95 and a specificity of 0.91. Moreover, we provide data that highlight IMPRESS's potential for liquid biopsies.

CONCLUSIONS:

We successfully created an innovative DNA methylation detection technique. By combining this method with a new multi-cancer biomarker panel, we developed a sensitive and specific multi-cancer assay, with potential use in liquid biopsies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores de Tumor / Islas de CpG / Metilación de ADN / Neoplasias Límite: Humans Idioma: En Revista: Br J Cancer Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores de Tumor / Islas de CpG / Metilación de ADN / Neoplasias Límite: Humans Idioma: En Revista: Br J Cancer Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido