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Inhibiting T-Cell-Mediated Rejection of the Porcine Meniscus Through Freeze-Thawing and Downregulating Porcine Xenoreactive Antigen Genes.
Chen, Rao; Zhao, Hailong; Ai, Liya; Zhang, Jiying; Jiang, Dong.
Afiliación
  • Chen R; Department of Sports Medicine, Institute of Sports Medicine, Peking University Third Hospital, Peking University, Beijing, P.R. China.
  • Zhao H; Beijing Key Laboratory of Sports Injuries, Beijing, P.R. China.
  • Ai L; Engineering Research Center of Sports Trauma Treatment Technology and Devices, Ministry of Education, Beijing, P.R. China.
  • Zhang J; School of Basic Medical Science, Peking University, Beijing, P.R. China.
  • Jiang D; Peking University International Cancer Institute, Beijing, P.R. China.
Cell Transplant ; 33: 9636897241273689, 2024.
Article en En | MEDLINE | ID: mdl-39180383
ABSTRACT
Immune rejection presents a significant challenge in xenogenic meniscal transplantation. Pigs are widely regarded as an advantageous tissue source for such transplants, with porcine GGTA1, CMAH, and B4GALNT2 being among the most common xenoreactive antigen (Ag) genes. While some studies have suggested that allogeneic meniscus (AM) transplants may exhibit immunoprivileged properties, our study observed slight immunological rejection has been observed following contact between human meniscal cells (HMCs) and human peripheral blood mononuclear cells (PBMCs). Given the limited systematic research on immune responses following xenograft meniscus transplantation, we established porcine meniscus transplantation (PMT) models to comprehensively assess the immunogenicity of porcine meniscus (PM) from both innate and adaptive immune perspectives. Our investigations confirmed that PMT beneath the epidermis led to innate cell infiltration into the xenografts and T-cell activation in local lymph nodes. T-cell activation upregulated the interleukin (IL)-17 signaling pathway, disrupting collagen organization and metabolic processes, thereby hindering PM regeneration. Using freeze-thaw treatment on PM alleviated T-cell activation post-transplantation by eliminating xenogenic DNA. In vitro findings demonstrated that gene editing in porcine meniscal cells (PMCs) suppressed human T-cell activation by downregulating the expression of xenoreactive Ag genes. These results suggest that GGTA1/CMAH/B4GALNT2 knockout (KO) pigs hold significant promise for advancing the field of meniscal transplantation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / Menisco / Galactosiltransferasas / Rechazo de Injerto Límite: Animals / Humans Idioma: En Revista: Cell Transplant Asunto de la revista: TRANSPLANTE Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / Menisco / Galactosiltransferasas / Rechazo de Injerto Límite: Animals / Humans Idioma: En Revista: Cell Transplant Asunto de la revista: TRANSPLANTE Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos