Your browser doesn't support javascript.
loading
In vitro differentiation of mouse pluripotent stem cells into corticosteroid-producing adrenocortical cells.
Oikonomakos, Ioannis; Tedesco, Melina; Motamedi, Fariba Jian; Peitzsch, Mirko; Nef, Serge; Bornstein, Stefan R; Schedl, Andreas; Steenblock, Charlotte; Neirijnck, Yasmine.
Afiliación
  • Oikonomakos I; Université Côte d'Azur, Inserm, CNRS, Institut de Biologie Valrose, 06108 Nice, France; Department of Internal Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
  • Tedesco M; Université Côte d'Azur, Inserm, CNRS, Institut de Biologie Valrose, 06108 Nice, France.
  • Motamedi FJ; Université Côte d'Azur, Inserm, CNRS, Institut de Biologie Valrose, 06108 Nice, France.
  • Peitzsch M; Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Carl Gustav Carus, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
  • Nef S; Department of Genetic Medicine and Development, University of Geneva, 1211 Geneva, Switzerland.
  • Bornstein SR; Department of Internal Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
  • Schedl A; Université Côte d'Azur, Inserm, CNRS, Institut de Biologie Valrose, 06108 Nice, France. Electronic address: schedl@unice.fr.
  • Steenblock C; Department of Internal Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany. Electronic address: charlotte.steenblock@ukdd.de.
  • Neirijnck Y; Université Côte d'Azur, Inserm, CNRS, Institut de Biologie Valrose, 06108 Nice, France.
Stem Cell Reports ; 19(9): 1289-1303, 2024 Sep 10.
Article en En | MEDLINE | ID: mdl-39178848
ABSTRACT
Directed differentiation of pluripotent stem cells into specialized cell types represents an invaluable tool for a wide range of applications. Here, we have exploited single-cell transcriptomic data to develop a stepwise in vitro differentiation system from mouse embryonic stem cells into adrenocortical cells. We show that during development, the adrenal primordium is embedded in an extracellular matrix containing tenascin and fibronectin. Culturing cells on fibronectin during differentiation increased the expression of the steroidogenic marker NR5A1. Furthermore, 3D cultures in the presence of protein kinase A (PKA)-pathway activators led to the formation of aggregates composed of different cell types expressing adrenal progenitor or steroidogenic markers, including the adrenocortical-specific enzyme CYP21A1. Importantly, in-vitro-differentiated cells responded to adrenocorticotropic hormone (ACTH) and angiotensin II with the production of glucocorticoids and mineralocorticoids, respectively, thus confirming the specificity of differentiation toward the adrenal lineage.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Células Madre Pluripotentes Límite: Animals Idioma: En Revista: Stem Cell Reports Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Células Madre Pluripotentes Límite: Animals Idioma: En Revista: Stem Cell Reports Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos