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Cascading CRISPR/Cas and Nanozyme for Enhanced Organic Photoelectrochemical Transistor Detection with Triple Signal Amplification.
Zhang, Lin; Hou, Lu; Cai, Hui-Hui; Sun, Bing; Han, De-Man; Chen, Feng-Zao.
Afiliación
  • Zhang L; School of Science, China University of Geosciences (Beijing), Beijing 100083, China.
  • Hou L; School of Pharmaceutical and Chemical Engineering, Taizhou University, Taizhou 318000, China.
  • Cai HH; School of Pharmaceutical and Chemical Engineering, Taizhou University, Taizhou 318000, China.
  • Sun B; School of Pharmaceutical and Chemical Engineering, Taizhou University, Taizhou 318000, China.
  • Han DM; School of Science, China University of Geosciences (Beijing), Beijing 100083, China.
  • Chen FZ; School of Pharmaceutical and Chemical Engineering, Taizhou University, Taizhou 318000, China.
Anal Chem ; 96(35): 14283-14290, 2024 Sep 03.
Article en En | MEDLINE | ID: mdl-39176473
ABSTRACT
Innovative signal amplification and transduction play pivotal roles in bioanalysis. Herein, cascading CRISPR/Cas and the nanozyme are integrated with electronic amplification in an organic photoelectrochemical transistor (OPECT) to enable triple signal amplification, which is exemplified by the miRNA-triggered CRISPR/Cas13a system and polyoxometalate nanozyme for OPECT detection of miRNA-21. The CRISPR/Cas13a-enabled release of glucose oxidase could synergize with peroxidase-like SiW12 to induce catalytic precipitation on the photogate, inhibiting the interfacial mass transfer and thus the significant suppression of the channel current. The as-developed OPECT sensor demonstrates good sensitivity and selectivity for miRNA-21 detection, with a linear range from 1 fM to 10 nM and an ultralow detection limit of 0.53 fM. This study features the integration of bio- and nanoenzyme cascade and electronic triple signal amplification for OPECT detection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transistores Electrónicos / MicroARNs / Técnicas Electroquímicas / Sistemas CRISPR-Cas / Glucosa Oxidasa Límite: Humans Idioma: En Revista: Anal Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transistores Electrónicos / MicroARNs / Técnicas Electroquímicas / Sistemas CRISPR-Cas / Glucosa Oxidasa Límite: Humans Idioma: En Revista: Anal Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos