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A compact microfluidic platform for rapid multiplex detection of respiratory viruses via centrifugal polar-absorbance spectroscopy.
Su, Ya; Jin, Xiangyu; Yang, Fan; Liu, Xuekai; Li, Fenggang; Zhao, Qingchen; Hou, Jialu; Zhang, Shuailong; Li, Hang; Huang, Guoliang; Fu, Rongxin.
Afiliación
  • Su Y; School of Medical Technology, Zhengzhou Academy of Intelligent Technology, Beijing Institute of Technology, Beijing, 100081, China; School of Biomedical Engineering, Tsinghua University, Beijing, 100084, China.
  • Jin X; School of Biomedical Engineering, Tsinghua University, Beijing, 100084, China.
  • Yang F; Beijing Advanced Innovation Center for Intelligent Robots and Systems, School of Mechatronical Engineering, Beijing Institute of Technology, Beijing, 10081, China.
  • Liu X; Clinical laboratory, Aerospace Center Hospital, Beijing, 100049, China.
  • Li F; Beijing Advanced Innovation Center for Intelligent Robots and Systems, School of Mechatronical Engineering, Beijing Institute of Technology, Beijing, 10081, China.
  • Zhao Q; School of Medical Technology, Zhengzhou Academy of Intelligent Technology, Beijing Institute of Technology, Beijing, 100081, China.
  • Hou J; School of Medical Technology, Zhengzhou Academy of Intelligent Technology, Beijing Institute of Technology, Beijing, 100081, China.
  • Zhang S; School of Integrated Circuits and Electronics, Beijing Institute of Technology, Beijing, 100081, China; Engineering Research Center of Integrated Acousto-opto-electronic Microsystems (Ministry of Education of China), Beijing Institute of Technology, Beijing, 100081, China; Chongqing Institute of Mic
  • Li H; School of Medical Technology, Zhengzhou Academy of Intelligent Technology, Beijing Institute of Technology, Beijing, 100081, China; Engineering Research Center of Integrated Acousto-opto-electronic Microsystems (Ministry of Education of China), Beijing Institute of Technology, Beijing, 100081, China
  • Huang G; School of Biomedical Engineering, Tsinghua University, Beijing, 100084, China. Electronic address: tshgl@tsinghua.edu.cn.
  • Fu R; School of Medical Technology, Zhengzhou Academy of Intelligent Technology, Beijing Institute of Technology, Beijing, 100081, China; Engineering Research Center of Integrated Acousto-opto-electronic Microsystems (Ministry of Education of China), Beijing Institute of Technology, Beijing, 100081, China
Talanta ; 280: 126733, 2024 Dec 01.
Article en En | MEDLINE | ID: mdl-39173249
ABSTRACT
Nucleic acid detection technology has become a crucial tool in cutting-edge research within the life sciences and clinical diagnosis domains. Its significance is particularly highlighted during the respiratory virus pandemic, where nucleic acid testing plays a pivotal role in accurately detecting the virus. Isothermal amplification technologies have been developed and offer advantages such as rapidity, mild reaction conditions and excellent stability. Among these methods, recombinase polymerase amplification (RPA) has gained significant attention due to its simple primer design and resistance to multiple reaction inhibitors. However, the detection of RPA amplicons hinders the widespread adoption of this technology, leading to a research focus on cost-effective and convenient detection methods for RPA nucleic acid testing. In this study, we propose a novel computational absorption spectrum approach that utilizes the polar GelRed dye to efficiently detect RPA amplicons. By exploiting the asymmetry of GelRed molecules upon binding with DNA, polar electric dipoles are formed, leading to precipitate formation through centrifugal vibration and electrostatic interaction. The quantification of amplicon content is achieved by measuring the residual GelRed concentration in the supernatant. Our proposed portable and integrated microfluidic device successfully detected five respiratory virus genes simultaneously. The optimized linear detection was achieved and the sensitivity for all the targets reached 100 copies/µL. The total experiment could be finished in 27 min. The clinical experiments demonstrated the practicality and accuracy. This cost-effective and convenient detection scheme presents a promising biosensor for rapid virus detection, contributing to the advancement of RPA technology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Amplificación de Ácido Nucleico Límite: Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Amplificación de Ácido Nucleico Límite: Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos