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Proteomic Profiling of Unannotated Microproteins in Human Placenta Reveals XRCC6P1 as a Potential Negative Regulator of Translation.
Li, Qiong; Liu, Fanrong; Ma, Xiaoyu; Chen, Feifei; Yi, Ziying; Du, Yangyang; Huang, Anxin; Zhao, Chenyang; Wang, Da; Chen, Yanran; Cao, Xiongwen.
Afiliación
  • Li Q; Department of Obstetrics and Gynecology, The First People's Hospital of Chenzhou, Chenzhou 423000, China.
  • Liu F; The First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
  • Ma X; Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, China.
  • Chen F; Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, China.
  • Yi Z; Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, China.
  • Du Y; Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, China.
  • Huang A; Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, China.
  • Zhao C; Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, Shanghai 200241, China.
  • Wang D; Department of Obstetrics and Gynecology, The First People's Hospital of Chenzhou, Chenzhou 423000, China.
  • Chen Y; The First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
  • Cao X; Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Navy Military Medical University, Shanghai 200003, China.
J Proteome Res ; 23(9): 4005-4013, 2024 Sep 06.
Article en En | MEDLINE | ID: mdl-39171377
ABSTRACT
Ribosome profiling and mass spectrometry have revealed thousands of previously unannotated small and alternative open reading frames (sm/alt-ORFs) that are translated into micro/alt-proteins in mammalian cells. However, their prevalence across human tissues and biological roles remains largely undefined. The placenta is an ideal model for identifying unannotated microproteins and alt-proteins due to its considerable protein diversity that is required to sustain fetal development during pregnancy. Here, we profiled unannotated microproteins and alt-proteins in human placental tissues from preeclampsia patients or healthy individuals by proteomics, identified 52 unannotated microproteins or alt-proteins, and demonstrated that five microproteins can be translated from overexpression constructs in a heterologous cell line, although several are unstable. We further demonstrated that one microprotein, XRCC6P1, associates with translation initiation factor eIF3 and negatively regulates translation when exogenously overexpressed. Thus, we revealed a hidden sm/alt-ORF-encoded proteome in the human placenta, which may advance the mechanism studies for placenta development as well as placental disorders such as preeclampsia.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Preeclampsia / Biosíntesis de Proteínas / Proteómica Límite: Female / Humans / Pregnancy Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Preeclampsia / Biosíntesis de Proteínas / Proteómica Límite: Female / Humans / Pregnancy Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos