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Effect of H2O2-VC degradation on structural characteristics and immunomodulatory activity of larch arabinogalactan.
Qi, Huimin; Tang, Shuo; Bian, Bin; Lai, Chenhuan; Chen, Yanan; Ling, Zhe; Yong, Qiang.
Afiliación
  • Qi H; Jiangsu Co-Innovation Center of Efficient Processing and Utilisation of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, China.
  • Tang S; Nanjing Institute of Comprehensive Utilization of Wild Plants, Nanjing, China.
  • Bian B; Jiangsu Co-Innovation Center of Efficient Processing and Utilisation of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, China.
  • Lai C; Jiangsu Co-Innovation Center of Efficient Processing and Utilisation of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, China.
  • Chen Y; Jiangsu Co-Innovation Center of Efficient Processing and Utilisation of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, China.
  • Ling Z; Jiangsu Co-Innovation Center of Efficient Processing and Utilisation of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, China.
  • Yong Q; Jiangsu Co-Innovation Center of Efficient Processing and Utilisation of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, China.
Front Bioeng Biotechnol ; 12: 1461343, 2024.
Article en En | MEDLINE | ID: mdl-39170060
ABSTRACT
The arabinogalactan in the representative softwood biomass of larch was degraded using an environmentally friendly hydrogen peroxide and vitamin C (H2O2-VC) system to improve its immunomodulatory activity. Through the H2O2-VC degradation mechanism, hydroxyl radicals are generated, which then target the hydrogen atoms within polysaccharides, resulting in the breaking of glycosidic bonds. Given the impact of oxidative degradation on polysaccharides, we identified three specific arabinogalactan degradation products distinguished by their arabinosyl side chain compositions. The primary structures of the degradation products were investigated using Fourier-transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. Congo red staining showed that the degradation products were absent in the triple-helix structure. The results of the in vitro immunological experiments indicated that an appropriate reduction in the molar ratio of arabinose to galactose enhanced the immunostimulatory effects on RAW 264.7 cells. In addition, the immunostimulatory pathway mediated by arabinogalactan was explored by toll-like receptor 4 (TLR4) inhibitor (TAK-242) These findings provide novel insights into the understanding of the relationship between the structure of arabinogalactan and its biological activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Bioeng Biotechnol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Bioeng Biotechnol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza